基于酶促反应的阿昔洛韦流动注射电化学发光分析法

基于阿昔洛韦与酶柱中的黄嘌呤氧化酶反应生成过氧化氢,继而与鲁米诺发生电化学发光反应的原理,建立了流动注射电化学发光测定阿昔洛韦的新方法,并将其应用于实际药品的检测.在pH为9.0的0.05mol/L的硼砂介质、激发电位为0.65V、鲁米诺浓度为5.00×10-4 mol/L、流速为1.90mL/min的优化条件下,测定阿昔洛韦的线性范围为0.56~22.5mg/L,线性相关系数为0.999 5,检出限为0.32mg/L,测定实际药品的相对标准偏差小于1.80%(n=9),加标回收率为95.5%~108%.

Flow-injection electrochemiluminescence analysis of aciclovir based on the enzymatic reaction

We report a new flow-injection electrochemiluminescence （ECL ） analytical method for the determination of aciclovir based on the enzymatic reaction of aciclovir by xanthine oxidase immobi-lized in a micro-column .When the sample flow s through the enzyme reactor ,the xanthine oxidase cat-alyzes aciclovir to produce hydrogen peroxide （H2 O2 ） .Followed by ECL reaction of H2 O2 with lumi-nol ,the acyclovir was determined in the sample .In the paper ,effects of the mobile phase ,electrolyte pH ,excitation potential ,and luminol concentration on the ECL intensity were investigated and opti-mized .The linear response of aciclovir concentrations ranged from 0 .56 to 22 .5 mg/L was obtained with a detection limit of 0 .32 mg/L .This method was successfully applied to detect the active ingre-dient of acyclovir in pharmaceutical preparations .The relative standard deviation was less than 1 .80% （n=9） and the recovery was in the range of 95 .5% ~108% for the determination of actual samples .