大熊猫父权确定的DNA证据

本文对成都大熊猫繁育研究基地,成都动物园,福州大熊猫研究中心及昆明动物园圈养繁殖的大熊猫１４胎次,１９只幼子,２５只次参配雄兽个体的被毛,血液,精液,肝,甲醛固定组织细胞,以及粪,尿等样品,采用同位素与荧光素标记基因探针作ＤＮＡ指纹图谱分析,得到了玫些有意义的结论。

DNA EVIDENCE IN THE PATERNITY DETERMINATION OF GIANT PANDA

he paternity determination of filial generation of artificial breeding Giant Panda including 19young babies and 25 "father" in 14 litters in Chengdu Research Base of Giant Panda Breeding,Chengdu Zoo,Fuzhou Giant Panda Research Centre and Kunming Zoo has been done by using the method of labelling oligonucleotide probe LZF-1 and JH 12. 6 with fluorescein and radioactive P,and the DNA were isolated from hairs, blood, semina, formalin-fixed testes,excrements and urine. (1 )There are the identical results of DNA fingerprints by detecting the hairs, blood,semina,formalin-fixed testes,excrements and urine of the same Giant Panda. (2)All the DNA hybrid bands of any young baby farrowed down by monogamy and polyandry including 2,3 and 4 males can been observed in their parents,and the true fathers of the 19 young babies have been determined. (3)The DNA fingerprints of 10 young babies in 5 pairs of twins are highly individual spe cific,and it has been known that the 5 pairs of twins are the freternal twins. In these fraternal twins 4 babiesborn in 1995 in 2 pairs of twins in Chengdu Research Base of Giant Panda Breeding and Chengdu Zoo have their respective fathers,and it has made showed that the filial generation of artificial breeding Giant Pandas havenotonly the fraternal twins but also the "twins of half blood". The results have provided the theoretical and applied basis for raising farrowing efficiency of female Giant Pandas by polyandry, mating or inseminating many times in an oestrus. (4 )The method of detecting the DNA fingerprints in the paternity determination of Ginat Pandas is sensitive and stable,it has solved the fear of disturbance in the rear for the mating ways of polyandry, mating or inseminating many times in an oestrus of Giant Panda. (5 ) The detecting results of oligonucleotide probe LZF-1 are better than that of JH 12. 6,and the method of labelling LZF--1 with fluorescein can totally replace that of labelling probe with radioactive P.