微生物与生物炭复合修复铬污染土壤的室内试验研究

以铬污染土为研究对象，通过浸出试验、Cr(VI)残留值试验、BCR连续提取试验研究了巴氏芽孢杆菌、生物炭及巴氏芽孢杆菌与生物炭复合对铬污染土壤的修复效果。结果表明：经过20 d修复后，上述添加剂均能够修复铬污染土壤，但菌液与生物炭复合的修复效果优于菌液和生物炭，且菌液的修复效果要优于生物炭；其中，当菌液浓度OD600 为1.0与生物炭浓度40 g?kg-1复合时，土壤浸出浓度和土壤中Cr(VI)含量分别从90 mg?L-1、270 mg?kg-1降低至1.08 mg?L-1、5.14 mg?kg-1，修复效果最好；同时，3种添加剂均提高了土壤的pH，均促使铬从弱酸态向可还原态和残渣态转化，而对可氧化态铬影响不大；由X射线光电子能谱分析（XPS）分析可知巴氏芽孢杆菌与生物炭复合修复铬污染土壤为混合吸附和还原的过程。

Experimental study on the remediation of chromium contaminated soil by microorganism and biochar

In order to study the remediation effects of Bacillus pasteurella, biochar and combination of Bacillus pasteurlla and biochar on chromium contaminated soil, by leaching test, Cr(VI) residual value test and BCR continuous extraction test. the results showed that the above additives could repair the chromium-contaminated soil after 20d restoration.but the remediation effect of the combination of bacterial solution and biochar was better than that of bacterial solution and biochar,and the remediation effect of bacterial solution was better than that of biochar; Among them, after the bacteria concentration of 1.0 and biochar of 40g/kg were treated, the leach concentration and Cr(VI) concent in soil were the best. the leach concentration and Cr(VI) content in the soil were reduced from 90 mg?L-1 and 270 mg?kg-1 to 1.08mg/L and 5.14mg/kg, At the same time, the three additives increased the pH of soil, promoted the transformation of chromium from weak acid state to reducible state and residual state, but had little effect on the oxidizable state of chromium; according to the analysis of XPS the mechanism of the combined of remediation of chromium contaminated soil by bacillus pasteuris and biochar is a process of mixed adsorption and reduction.