黄浆水与木薯粉混合发酵高浓度乙醇的发酵条件

以黄浆水与木薯粉混合同步糖化发酵高浓度乙醇，应用Plackett—Burman设计对底物浓度、尿素、糖化酶、液化酶、MgSOa、pH值、CaCl2、KH2PO4,8个因素进行2水平12次试验，研究发酵的最佳条件。结果发现，底物浓度、尿素、糖化酶和KH。PO。的添加量是影响发酵的重要因素，最佳发酵条件是底物浓度38％、尿素0．15％、糖化酶1．45AGU／g、KH2PO4 0．07％。在最佳发酵条件下，乙醇浓度达到17．21％（v／v）。

Techniques for Fermentating High-concentration Ethanol from Mixing Yellow Serofluid and Cassava

The conditions of simultaneous saccharification and fermentation by mixing yellow serofluid and cassava flour were investigated for producing high-concentration ethanol.The important parameters were screened by Plackett-Burman design,and subsequently the path of steepest ascent was used to approach to the biggest region of ethanol production.Finally,the optimum values of parameters were obtained by orthogonal tests.The experimental results showed the optimum technical conditions as follows:Substrate concentration 38%,CO(NH2)2 0.15%,Dextrozyme 1.45 AGU/g substrate,KH2PO4 0.07%,under which the ethanol reached 17.21%(V/V) after 48 h fermentation at 32°C and 100 r/min.