| 胎盘绒毛细胞的培养及其基因组DNA的分离纯化 |
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| 引用本文： | 吴文莲,李建吾.胎盘绒毛细胞的培养及其基因组DNA的分离纯化[J].四川大学学报(自然科学版),1993,30(4):531-533. |
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| 作者姓名： | 吴文莲 李建吾 |
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| 作者单位： | 四川大学生物系
(吴文莲，李建吾，郑远旗)，四川大学生物系(张静) |
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| 摘 要： | 从采集到的微量胎盘绒毛，经过组织细胞培养，获得了大量的绒毛细胞，对其进行了基因组ＤＮＡ的分离纯化，提取到足够量的ＤＮＡ。经过０．８％琼脂糖凝胶电泳，结果显示出完整的绒毛细胞ＤＮＡ带，得到了典型的大分子ＤＮＡ电泳图谱。
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| 关 键 词： | 胎盘绒毛 绒毛细胞 DNA |
THE CULTURE OF CHORIONIC VILLUS CELL AND THE ISOLATION OF ITS GENOME DNA |
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| Wu Wenlian Li Jianwu Zheng Yuanqi Zhang Jin.THE CULTURE OF CHORIONIC VILLUS CELL AND THE ISOLATION OF ITS GENOME DNA[J].Journal of Sichuan University (Natural Science Edition),1993,30(4):531-533. |
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| Authors: | Wu Wenlian Li Jianwu Zheng Yuanqi Zhang Jin |
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| Institution: | Department of Biology |
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| Abstract: | In this experiment, a method of culture of chorionic villus cell was groped. Using this method,a great deal of villus cells were obtained from a few villus cell. We isolated the genome DNA from them. On the pattern of agarose gel electrophoresis, the villus genome DNA showed one complete band,which was typical macromolecular DNA electrophoretic pattern. |
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| Keywords: | chorionic villus villus cell villus genome DNA |
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