| Cu2＋猝灭茜素红荧光法检测谷胱甘肽 |
| |
| 引用本文： | 牛淑妍,沈晓彤,崔欢欢,孙娟.Cu2＋猝灭茜素红荧光法检测谷胱甘肽[J].青岛化工学院学报(自然科学版),2014(3):221-224. |
| |
| 作者姓名： | 牛淑妍 沈晓彤 崔欢欢 孙娟 |
| |
| 作者单位： | 青岛科技大学化学与分子工程学院,山东青岛266042 |
| |
| 基金项目： | 国家自然科学基金项目（21075073）;山东省自然科学基金重点项目（ZR2010BZ006） |
| |
| 摘 要： | 茜素红（ARS）与硼酸（H3BO3）之间能发生相互作用形成黄色配合物,使ARS的荧光增强,Cu2＋可以猝灭茜素红在Britton-Robison（BR）缓冲溶液中的荧光。加入谷胱甘肽（GSH）后,由于GSH所带的巯基（—SH）和Cu2＋发生络合,使体系的荧光强度增大,据此可以快速的测定GSH的含量。在pH 6.3的BR缓冲溶液中,茜素红-Cu2＋体系荧光强度的增强值与GSH的浓度在1.0×10-5～1.9×10-4 mol·L-1范围内呈线性关系,检测限为1.0×10-5 mol·L-1。
|
| 关 键 词： | 谷胱甘肽 铜离子 茜素红 荧光光谱 |
Detection of Glutathione by Copper Ions Quenching Fluorescence of Alizarin Red |
| |
| NIU Shu-yan,SHEN Xiao-tong,CUI Huan-huan,SUN Juan.Detection of Glutathione by Copper Ions Quenching Fluorescence of Alizarin Red[J].Journal of Qingdao Institute of Chemical Technology(Natural Science Edition),2014(3):221-224. |
| |
| Authors: | NIU Shu-yan SHEN Xiao-tong CUI Huan-huan SUN Juan |
| |
| Institution: | (College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China) |
| |
| Abstract: | The alizarin red（ARS）interacted with H3BO3to form a yellow complex,which resulted in the fluorescence enhancement of the ARS.Cu2＋could quench the fluorescence of ARS.The reaction of glutathione（GSH）with Cu2＋and the effect of GSH on the fluorescent systems,i.e.ARS-Cu2＋were studied.The mercapto（-SH）of GSH added into the system had a firmer combination with Cu2＋,which would wrested the Cu2＋combined with H3BO3,resulting in the recovery of fluorescence.As a result,GSH was detected rapidly.In the Britton-Robison buffer（pH 6.3）solution,agood linear relationship was obtained from 1.0×10-5 to 1.9×10-4 mol·L-1 for GSH,the detection limit was 1.0×10-5 mol·L-1. |
| |
| Keywords: | glutathione copper ions alizarin red fluorescence spectrum |
| 本文献已被 维普 等数据库收录！ |
|
