| 麸曲糖化酶活力测定方法探讨 |
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| 引用本文： | 罗惠波,;王毅,;边名鸿,;杨晓东,;杨建勤.麸曲糖化酶活力测定方法探讨[J].四川理工学院学报(自然科学版),2014(3):6-9. |
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| 作者姓名： | 罗惠波 ;王毅 ;边名鸿 ;杨晓东 ;杨建勤 |
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| 作者单位： | [1]四川理工学院生物工程学院,四川自贡643000; [2]酿酒生物技术及应用四川省重点实验室,四川自贡643000; [3]江安县工业园区管理委员会,四川江安644200; [4]成都新忆坊商贸有限公司,成都610036 |
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| 基金项目： | 酿酒生物技术及应用四川省重点实验室开放基金项目（NJ2010-05;NJ2011-05;NJ2013-03） |
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| 摘 要： | 糖化酶活力是制曲过程中的一个重要指标。建立了3,5-二硝基水杨酸(DNS)结合连续流动化学分析仪(API)检测麸曲糖化酶活力的方法。结果表明:通过t检验,次碘酸盐法、DNS法和DNS优化法测定糖化酶活力均无显著性系统误差,DNS优化法的误差值最低为13.1 U/g,RSD值为1.97%。通过F-检验双样本方差分析,DNS优化法较快速滴定法、DNS法测定麸曲糖化酶活力的精密度均有显著性提高,且具有简便、快捷的特点。
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| 关 键 词： | 麸曲 连续流动化学分析仪(API) 3 5-二硝基水杨酸(DNS) 糖化酶 |
Study on the Determination of Glucoamylase Activity of Koji |
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| LUO Huibo;WANG Yi;BIAN Minghong;YANG Xiaodong;YANG Jianqin.Study on the Determination of Glucoamylase Activity of Koji[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2014(3):6-9. |
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| Authors: | LUO Huibo;WANG Yi;BIAN Minghong;YANG Xiaodong;YANG Jianqin |
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| Institution: | LUO Huibo,WANG Yi,BIAN Minghong,YANG Xiaodong,YANG Jianqin( 1. School of Biotechnology Engineering, Sichuan University of Science ＆ Engineering, Zigong 643000, China ; 2. Liquor Making Bio-Technology ＆ Application of Key Laboratory of Sichuan Province, Zigong 643000, China ; 3. Industrial Park Management Committee of Jiangan County, Jiangan 644200, China; 4. New Recalling Square-commerce Co. Ltd of Chengdu 610036, China) |
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| Abstract: | One of the important indexs of Koji preparation is glucoamylase activity. A method that combined 3,5-Dinitrosalicylic acid（DNS） with American Continuous-Flow Analysis-CFA（ API） is established to detect the glucoamylase activity. The results indicate that,the t tests of glucoamylase activity by hypoiodite,DNS and improved DNS all have no significance systematic errors. The lowest error value of improved DNS is 13. 1U /g,RSD is 1. 97%. Through F test double sample variance analysis,glucoamylase activity determination by improved DNS has higher precision than that by rapid titration and DNS,and the improved DNS has the traits of simple and rapid operation. |
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| Keywords: | Koji Continuous-Flow Analysis-CFA（API） 3 5-Dinitrosalicylic acid（DNS） glucoamylase |
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