| In Vitro Cytotoxicity and Protein Drug Release Properties of Chitosan/Heparin Mlcrospheres |
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| 作者姓名： | 贺庆 敖强 王爱军 公衍道 赵南明 张秀芳 |
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| 作者单位： | State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| 基金项目： | Supported by the National Key Basic Research and Development （973） Program of China （No. 2005CB623905） and the Tsinghua- Yue-Yuen Medical Science Fund |
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| 摘 要： | Chitosan/heparin microspheres were prepared using the water-in-oil emulsification solvent evaporation technique. The microsphere diameters were controlled by selecting the fabrication process parameters. Scanning electron micrographs showed that the chitosan/heparin microspheres were regular and the surface morphology was smooth. Fourier transform infrared showed that the chitosan amino groups reacted with heparin carboxylic groups to form acylamides in the microspheres. Analysis of the microsphere cytotoxicity showed that they had no cytotoxic effect and behaved very similar to the negative control （polystyrene）. To analyze the protein drug release profiles of the microspheres, bovine serum albumin was loaded as a model drug into the microspheres and released in vitro. Marked retardation was observed in the BSA release profiles. The results show that chitosan/heparin microspheres may provide a useful controlled release protein drug system for used in pharmaceutics.
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| 关 键 词： | 脱乙酰壳多糖 肝素 微球 细胞毒性 药品 |
| 修稿时间： | 2005-09-21 |
In Vitro Cytotoxicity and Protein Drug Release Properties of Chitosan/Heparin Mlcrospheres |
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| HE Qing, AO Qiang , WANG Aijun , GONG Yandao , ZHAO Nanming , ZHANG Xiufang.In Vitro Cytotoxicity and Protein Drug Release Properties of Chitosan/Heparin Mlcrospheres[J].Tsinghua Science and Technology,2007,12(4):361-365. |
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| Authors: | HE Qing AO Qiang WANG Aijun GONG Yandao ZHAO Nanming ZHANG Xiufang |
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| Institution: | State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | |
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| Keywords: | chitosan heparin microspheres cytotoxicity controlled release |
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