Eine Methode zur Trockenfixation grossflächiger Kryostatschnitte |
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Authors: | H. Möllmann D. H. Niemeyer K. Wilp H. Alfes G. Henke |
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Affiliation: | (1) Anatomisches Institut und Institut für Pharmazeutische Chemie der Universität, D-44 Münster, Deutschland |
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Abstract: | Summary By exposure to formaldehyde vapor after freeze-drying large cryostat sections are brought into a stable form. These sections can be used for documentation and may be compared easily with autoradiograms made of the same slices. Furthermore, a method is shown of evaluating these sections with a thin-layer scanner and a 2-flow counter, or by measuring quantitatively the radioactivity of single, excised organs or parts of tissues after automatic combustion by liquid scintillation counting. |
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