| 不同海域中国花鲈的细胞色素b序列的遗传分析 |
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| 引用本文： | 刘明月,蒋琦辰,杨家新.不同海域中国花鲈的细胞色素b序列的遗传分析[J].南京师大学报,2010,33(1). |
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| 作者姓名： | 刘明月 蒋琦辰 杨家新 |
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| 作者单位： | 南京师范大学生命科学学院; |
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| 基金项目： | 江苏省海洋生物技术开放实验室项目(编号:2007HS007) |
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| 摘 要： | 从辽宁大连、河北黄骅、山东青岛、江苏盐城、浙江宁波、广西北海等6个沿海地区分别采集野生中国花鲈共44尾,通过测定花鲈细胞色素b序列,获得876 bp长度的序列,检测到的变异位点26个,22个单倍型,A、C、G、T的碱基组成分别为22.6%,33.1%,16.4%,28.0%.各群体内的遗传差异从0.1%~1%,群体间的遗传差异从0.2%~1%.构建NJ树,分为两大群,取自北海的花鲈单独成群,其它地区的花鲈合为另一分支.
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| 关 键 词： | 中国花鲈 线粒体DNA 细胞色素b 序列分析 |
Analysis on Mitochondrial DNA Cytochrome b Gene of Lateolabrax japonicas From Different Seas |
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| Liu Mingyue,Jiang Qichen,Yang Jiaxin.Analysis on Mitochondrial DNA Cytochrome b Gene of Lateolabrax japonicas From Different Seas[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(1). |
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| Authors: | Liu Mingyue Jiang Qichen Yang Jiaxin |
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| Institution: | School of Life Science/a>;Nanjing Normal University/a>;Nanjing 210046/a>;China |
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| Abstract: | The mitochondrial DNA cytochrome b(Cytb) gene of Lateolabrax japonicas samples collected form Dalian(DL),Huanghua(HH),Qingdao(QD),Yancheng(YC),Ningbo(NB)and Beihai(BH) were amplified with PCR technique and DNA sequencing.The results showed that 26 nucleotide sites were variable among 876bp length of homologous sequence.A total of 22 haplotypes found in all 44 individuals.The average A,C,G and T contents in Cytb gene were 22.6%,33.1%,16.4%and 28.0%,respectively.Sequence divergence of Cytb of Lateolabrax japo... |
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| Keywords: | Lateolabrax japonicas mitochondrial DNA cytochrome b (Cytb) sequencing |
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