电针对AD模型大鼠海马神经干细胞Nestin及学习记忆能力的影响

目的:探讨电针对淀粉样肽(-AP)所致阿尔茨海默病(AD)模型大鼠脑内Nestin表达的影响,并对其保护作用提供理论依据.方法:取健康的SD大鼠40只,随机分为正常组、假手术组、模型组和电针组.用A 1-40微量注射至大鼠双侧Meynert基底核,建立AD模型,用电针进行治疗.于治疗后用Morris水迷宫检测大鼠的学习记忆能力,用免疫组化染色法检测Nestin阳性神经细胞数的表达.结果:①学习记忆观察:电针组的逃避潜伏期较模型组明显缩短,大鼠在原平台象限游泳时间占整个游泳时间的百分比显著升高,穿过原平台所在位置的次数明显增加(<0.01).②Nestin免疫组化检测:电针组大鼠海马Nestin阳性神经元数量在治疗组表达最强,与模型组相比具有显著性差异(<0.01).结论:电针能增强AD模型大鼠海马神经干细胞Nestin的表达,提高AD大鼠的学习记忆能力,其机制尚需讨论.

Effects of Electroacupuncture on AD Model Rat Hippocampus Neural Stem Cells Nestin and Impact on Learning and Memory Capacity

Objective： To investigate the effect by electroacupuncture to β - amyloid peptide （β-AP） caused Alzheimer＇s disease （AD） rat model on brain expression of Nestin, and provide a theoretical basis for its protection effect. Methods： Admission healthy SD rats 40, randomly divided into normal control group, sham-operated control group, the model group and electroacupuncture group. Using A 13,40 with microinjection to bilateral Meynert basal ganglia of rats, to establish AD model, using electroacupuncture treatment, after treatment uses Morris water maze to investigate the learning ability and memory of rats, and uses immunohistochemical staining to detect the number of Nestin positive nerve cells. Results： （1）The learning and memory observation： All the escape latency of the treatment group was significantly shorter than the model, rats swimming in the original platform quadrant in all the swimming time were significantly improved, the times crossing original platform location increased significantly （P 〈 0.01）. （2）Immunohistochemical detection of Nestin： the rat hippocampal Nestin positive neurons of the electroacupuncture group is the highest, compare to model group there is statistical significance （P 〈 0.01）. Conclusion： The electroacupuncture will enhance the Nestin expression in hippocampus neural stem cells of AD model rats, improve learning ability and memory in AD rats, the mechanism needs further research