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| 酶辅助超声波法提取连翘叶总黄酮工艺 | |
| 引用本文： | 吴艳芳,王新胜,尹延彦,陈蓉,李方方. 酶辅助超声波法提取连翘叶总黄酮工艺[J]. 河南科技大学学报(自然科学版), 2012, 0(1): 96-99,10 |
| 作者姓名： | 吴艳芳 王新胜 尹延彦 陈蓉 李方方 |
| 作者单位： | 新乡医学院药学院;河南科技大学化工与制药学院;南京中医药大学药学院 |
| 基金项目： | 河南省科技创新杰出青年基金项目(104100510016);新乡医学院省级重点学科开发项目(ZD200968) |
| 摘 要： | 采用酶辅助超声波法提取连翘叶总黄酮,在超声波条件固定的情况下,研究了酶解时间、酶解液pH值、酶解温度和酶用量对连翘叶总黄酮的影响。获得的优化条件为:酶解时间1.5 h,酶解温度40℃,酶量3 mg/g,最适pH值为5.0。在此条件下测定的连翘叶总黄酮含量为(26.53±0.68)%。试验结果表明:酶辅助超声波法提取连翘叶总黄酮是一种行之有效的方法。 |
| 关 键 词： | 酶法 超声提取 连翘叶 总黄酮 |
Extraction Technology of Total Flavones from Fructus Forsythia Leaves by Enzymetic-assisted Ultrasonic Methods |
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| WU Yan-Fang,WANG Xin-Sheng,YIN Yan-Yan,Chen Rong,LI Fang-Fang. Extraction Technology of Total Flavones from Fructus Forsythia Leaves by Enzymetic-assisted Ultrasonic Methods[J]. Journal of Henan University of Science & Technology:Natural Science, 2012, 0(1): 96-99,10 | |
| Authors: | WU Yan-Fang WANG Xin-Sheng YIN Yan-Yan Chen Rong LI Fang-Fang |
| Affiliation: | 1.School of Pharmaceutical,Xinxiang Medical University,Xinxiang 453003,China;2.Chemical & Pharmaceutical School, Henan University of Science & Technology,Luoyang 471003,China;3.Pharmaceutical College,Nanjing University of Chinese Medicine,Nanjing 210046,China) |
| Abstract: | Using fructus forsythia leaves as material,the total flavones were extracted by enzymetic-assisted ultrasonic method.With the ultrasonic conditions fixed,the influences of pH of enzyme solution,incubation time,incubation temperature and amount of enzyme on total flavones were investigated.The optimum conditions obtained are incubation time 1.5 h,incubation temperature 40 t,amount of enzyme 3 mg/g,pH of enzyme solution 5.0.Under above optimum conditions,the yield of the total flavones is(26.53±0.68)%.Enzymeticassisted ultrasonic method may be used as a effective and promising technique for the extraction of flavones from this plant. |
| Keywords: | Enzymetic-assisted method Ultrasonic extraction Fructus forsythia leaf Total flavonses |
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