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| 研究聚ADP核糖基化作用与细胞恶变关系的双基因真核表达重组体的构建 | |
| 引用本文： | 杨其伟,张学,陈雅文,董元舒,陈德风. 研究聚ADP核糖基化作用与细胞恶变关系的双基因真核表达重组体的构建[J]. 南开大学学报(自然科学版), 1997, 0(1) |
| 作者姓名： | 杨其伟 张学 陈雅文 董元舒 陈德风 |
| 作者单位： | 南开大学分子生物学研究所!天津，300071 |
| 摘 要： | 本文将聚ADP核糖聚合酶基因1．4kb部分序列反向插入真核表达载体pMAMneo和pSMG中，同时12位密码子突变的活化ras癌基因也一同克隆到上述载体中，从而获得具有不同真核基因筛选标记的双基因真核表达重组体pMAMneo－Cl．4－T24，pMAMneo－Cl.4－arT24，及pSMG－Cl．4-T24，上述质粒的构建成功为研究聚ADP核糖基化作用与细胞恶变的关系提供了一种新的分子模型． |
| 关 键 词： | 聚ADP核糖基化水平 癌基因 细胞恶变 质粒构建 |
CONSTRUCTION OF DUAL GENE EUKARYOTIC EXPRESSION RECOMBINANTS FOR STUDY OF RELATIONSHIP BETWEEN POLY(ADP-RIBOSYL)ATION AND TUMORIGENESIS |
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| Yang Qiwei, Zhang Xue, Chen Yawen, Dong Yuanshu, Chen Defeng. CONSTRUCTION OF DUAL GENE EUKARYOTIC EXPRESSION RECOMBINANTS FOR STUDY OF RELATIONSHIP BETWEEN POLY(ADP-RIBOSYL)ATION AND TUMORIGENESIS[J]. Acta Scientiarum Naturalium University Nankaiensis, 1997, 0(1) | |
| Authors: | Yang Qiwei Zhang Xue Chen Yawen Dong Yuanshu Chen Defeng |
| Abstract: | In this paper,1.4kb partial cDNA for poly(ADP-ribose)polymerase was inserted in opposite orientation into mammalian expression vectors,pMAMneo and pSMG respective-ly.Besides,activated ras oncogene was also cloned into the same vectors.The final con-structs pMAMneo-Cl.4-T24,pMAMneo-Cl.4-arT24,and pSMG-Cl.4-T24 give a new molecular model for study on relationship hetween poly (ADP-ribosyl) ation and tumori-genesis. |
| Keywords: | level of poly (ADP-ribosyl) ation oncogene malignant transformation of cells plasmid construction |
| 本文献已被 CNKI 等数据库收录！ | |