三疣梭子蟹病原菌检测与分析

分离患病三疣梭子蟹样本中的病原菌,对分离菌株进行形态特征、理化特性和分子生物学鉴定。采用普通营养琼脂及zobell2216E海水营养琼脂分离患病样本中的病原菌。采用细菌常规生化鉴定方法,了解细菌的形态和生理生化特性。采用16SrRNA和gyrB基因序列同源性比对,进一步明确该病原菌的分类学地位。采用人工感染实验的方法,证明分离菌株是三疣梭子蟹致病病原菌。该菌革兰氏阴性,氧化酶阳性,葡萄糖发酵型,在低于10 g/L的NaCl胨水中不生长。16SrRNA和gyrB基因与需钠弧菌(Vibrio natriegen)同源序列具有99%的相似性。分离菌株对三疣梭子蟹有明显的致病性。需钠弧菌是引起江苏省连云港市赣榆县石桥镇九里村三疣梭子蟹严重死亡的病原菌。

Detection and Analysis of Pathogenic Bacteria from Diseased Portunus trituberculatus

In order to obtain and identify some pathogenic organisms isolated from the diseased Portunus trituberculatus in the crabs ponds of Ganyu county,Lianyungang city,Jiangsu province,morphological characteristics,physical and chemical properties,as well as molecular biology were analyzed.The dominant bacterial strain was Gram-negative,oxidase positive,a fermented glucose produced acid and fail to grow on 10 g/L NaCl medium.Meanwhile,16S rRNA and gyrB genes analysis showed that isolation presented 0.99 sequences similarity coefficients with Vibrio natriegen.In addition,isolation demonstrated obvious pathogenicity with Portunus trituberculatus.From all above studies,it is evident that the isolation was Vibrio natriegen of the genus Vibrio.