发根农杆菌Ri质粒诱导的黄芪发状根根冠细胞亚显微结构研究

对膜荚黄芪发状根根冠细胞的亚显微结构进行研究发现：发状根根尖没有分化出典型的根冠结构；根冠平衡囊细胞中淀粉分布无极性，散地细胞质中；细胞中未发现粗面内质网而仅有滑面内质网的存在；高尔基体分泌功能不旺盛，无肥大的潴泡；细胞壁较薄，纵向壁略加厚，其间无粘液积累；根冠外周细胞逐渐老化，但细胞壁不溶解，老化的细胞不脱落，且根冠细胞层数不增加。

Subcellular structures of the root cap cells of hairy roots in astragalus membranaceus induced by infection of Agrobacterium rhizogenes

The hairy roots of Astragalus membranaceus were induced by an introduction of Agrobacterium rhizogenes pRi 15834, pGSGlucl. It was shown that no typical root cap had been developed and the changes of subcellular structures were as follows: random distribution of amyloplasts in the statocyst; only sER was present the cells but no rER was found; little secretion of Golgi apparatuses; no mucus formation between cell walls.The statocyst was thin-walled and formed nonsecretory cell similar to that of the meristematic and elongating regions. The root cap cells didn't fall off during growth, and no increase of cell layers was observed. We suggest that these changes are related to the high production of secondary metabolite of hairy roots.