The effects of diverse agents (Ca++ and K++ ionophores, organomercurials, dithiols, colchicine, and cytochalasin B) on the maturation and differentiation without cleavage in Chaetopterus eggs] |
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Authors: | J Brachet S Denis-Donini |
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Abstract: | Induction of maturation in Chaetopterus oocytes requires the presence of Ca++ ions in the medium, but differentiation without cleavage can proceed in the absence of this cation. The Ca++ ionophore A 23187 induces both maturation and the cortical reaction provided that Ca++ ions are present in the medium differentiation without cleavage may follow. Valinomycin slowly induces germinal vesicle breakdown, which is followed by a sharp segregation between hyaloplasm and yolk. PHMPS, but not DTT, induces maturation. Differentiation without cleavage is more sensitive to colchicin than to cytochalasin B. |
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