| ICBP90介导PI3K/AKT通路阻断剂LY294002抑制Jurkat T细胞增殖 |
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| 引用本文： | 郭中峰,邢飞跃,方志远.ICBP90介导PI3K/AKT通路阻断剂LY294002抑制Jurkat T细胞增殖[J].暨南大学学报,2009,30(4). |
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| 作者姓名： | 郭中峰 邢飞跃 方志远 |
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| 作者单位： | 1. 暨南大学生命科学技术学院组织移植与免疫实验中心,广东,广州,510632
2. 法国路易斯·巴斯德大学国家健康医学研究所,法国67404 |
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| 基金项目： | 国家自然科学基金资助项目，广东省自然科学基金资助项目 |
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| 摘 要： | 目的:探讨磷脂酰肌醇3激酶/丝氨酸苏氨酸蛋白激酶B(PI3K/AKT)信号通路特异性抑制剂LY294002在JurkatT细胞增殖中的作用.方法:以急性T细胞白血病胞(Jurkat T 细胞)为模型,PD98059和阿霉素为阳性对照,在倒置显微镜下观察LY294002对Jurkat T细胞的集落形成的影响,四甲基偶氮唑蓝(MTT)检测LY294002处理后Jurkat T细胞的增殖,流式细胞术检测LY294002处理后Jurkat T细胞周期的变化,Western blotting方法检测Jurkat T细胞中ICBP90蛋白的表达以确定其与LY294002抑制Jurkat T细胞增殖的关系.结果:LY294002能够显著抑制Jurkat T细胞的集落形成和增殖,使Jurkat T细胞停滞于G2/M 期,导致Jurkat T细胞ICBP90蛋白的表达显著降低,LY294002与阿霉素联合用药可产生一定的协同效应. 结论:LY294002通过下调Jur-katT细胞ICBP90蛋白的表达,抑制Jurkat T细胞增殖.
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| 关 键 词： | 2-(4-吗啉基)-8-苯基-4氢-1-苯并吡喃-4-(LY294002) CCAAT盒结合蛋白(ICBP90) 增殖 细胞周期 急性T细胞白血病细胞(Jurkat T细胞) |
Inhibitor LY294002 of PI3K/AKT signal pathway mediated by ICBP90 inhibits proliferation of Jurkat T cell |
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| GUO Zhong-feng,XING Fei-yue,FANG Zhi-yuan,Christian Bronner.Inhibitor LY294002 of PI3K/AKT signal pathway mediated by ICBP90 inhibits proliferation of Jurkat T cell[J].Journal of Jinan University(Natural Science & Medicine Edition),2009,30(4). |
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| Authors: | GUO Zhong-feng XING Fei-yue FANG Zhi-yuan Christian Bronner |
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| Institution: | 1.Institute of Tissue Transplantation and Immunology;College of Life Science and Technology;Jinan University;Guangzhou 510632;China;2.Institute National de la Sante et de la Recherche Medicale;Universite Louis Pasteur;Strasbourg 67404;France |
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| Abstract: | Aim:To investigate the role of LY294002 which is the inhibitor of PI3K/Akt signal pathway in proliferation of Jurkat T and expression level of ICBP90. Methods: Jurkat T cells were used as a suitable model for this study.The positive control was Adriamycin and PD98059.Inverted microscope was used to observe the colony formation of Jurkat T cells.The proliferation of cells treated by LY294002,PD98059,Adriamycin,LY294002 combining with Adriamycin and PD98059 combining with Adriamycin,respectively,was checked b... |
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| Keywords: | LY294002 ICBP90 proliferation cell cycle Jurkat T cell |
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