| 从血清乳酸脱氨酶的分析探讨大土北鸭的杂优效应 |
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| 引用本文： | 肖景霖,施超欧,张松踪. 从血清乳酸脱氨酶的分析探讨大土北鸭的杂优效应[J]. 厦门大学学报(自然科学版), 1990, 0(1) |
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| 作者姓名： | 肖景霖 施超欧 张松踪 |
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| 作者单位： | 厦门大学生物学系,厦门大学生物学系,厦门大学生物学系 宁波制药厂 |
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| 摘 要： | 对三种家鸭血清LDH活力测定的结果,发现其总活力和比活力的大小依次为大土北鸭>金定鸭>北京鸭。采用等电聚焦电泳,对家鸭血清中LDH同工酶进行分析,得到电泳区带分别在 18～20条之间,可以看到有2个峰簇区,同时表现出正极端的不均一性。测得等电点范围为 pH4.8～8.8,但几乎集中在pH6.0～8.0,试验表明:大土北鸭LDH生化特性明显偏向金定鸭。结果为杂优分析提供了有意义的分子学基础。
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| 关 键 词： | 家鸭 乳酸脱氢醇 等电聚焦 |
Heterosis Effection of "Big-Tubei" Duck by Analyzing Serum LDH |
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| Xiao Jinglin Shi Chaoou Zhang Songzong. Heterosis Effection of "Big-Tubei" Duck by Analyzing Serum LDH[J]. Journal of Xiamen University(Natural Science), 1990, 0(1) |
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| Authors: | Xiao Jinglin Shi Chaoou Zhang Songzong |
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| Affiliation: | Dept. of Biol. |
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| Abstract: | From the result of activities of three domestic duck serum LDH. it can be found that total activity and specific activity sequence is "Big-Tubei" duck>"Jinding" duck>"Beijing" duck. The above three LDH isozymes are about 18-20 bands in the electropherogram respectively by using isoelectric focusing. These bands form two peak-clump regions. At the same time, electro pherogram appear different in cathode. Their isoelectric points are in the range of pH 4.8-8.8. but almost concentrate on pH 6.0-8.0 The experiment show that the biochemical features of "Big-Tub ei" duck's LDH isozyme obviously tend to "Jinding* duck. These results provide molecular basis for heterosis analysis. |
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| Keywords: | Domestic duck. Lactate dehydrogenase. Isoelectric focusing |
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