| 靶向hnRNP K基因siRNA的筛选及其对精原细胞存活的影响 |
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| 作者单位： | ;1.信阳师范学院生命科学学院 |
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| 摘 要： | 利用免疫细胞荧光、RNA干涉(RNA interfere,RNAi)、qRT-PCR、Western blot及细胞计数等方法,设计合成三对小分子干扰RNA(small interfering RNA,siRNA)(siRNA-hnRNP K-1,2,3),对小鼠精原细胞中内源性的不均一性核糖核蛋白K(Heterogeneous ribonucleoproteins K,hnRNP K)进行敲低并对其沉默效率进行筛选,研究其对小鼠精原细胞存活的影响.结果表明,siRNA-hnRNP K-3对小鼠精原细胞具有80%以上的沉默效率,转染后发现细胞数目明显减少,说明沉默hnRNP K基因的表达对小鼠精原细胞的存活有显著影响.
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| 关 键 词： | 不均一性核糖核蛋白K 精原细胞 小鼠 精子发生 |
Screening of siRNA Targeted for hnRNP K Gene and Its Effect on the Spermatogonium Survival |
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| Institution: | ,College of Life Sciences,Xinyang Normal University |
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| Abstract: | Through the immune cell fluorescence,RNAi(RNA interfere),qRT-PCR,Western blot and cell counting methods,three pairs of siRNA(small interfering RNA)(siRNA-hnRNP K-1,2,3)were designed and synthesed to screen the silence efficiency that the endogenous hnRNP K of spermatogonium in mice were knockdown,and then studied the effect on spermatogonium survival.Results showed that the siRNA-hnRNP K-3had silence efficiency more than 80%,and the cell number decreased significantly from further transfection to spermatogonium in mice.The results showed that the hnRNP K gene knockdown had a significant effect on the survival of spermatogonium in mice. |
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| Keywords: | hnRNP K spermatogonium mouse spermatogenisis |
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