Porcine factor V: cDNA cloning, gene mapping, three-dimensional protein modeling of membrane binding sites and comparative anatomy of domains |
| |
| Authors: | D R Grimm M B Colter M Braunschweig L J Alexander P J Neame H K W Kim |
| |
| Institution: | (1) Shriners Hospital for Children, Center for Research in Skeletal Development and Pediatric Orthopedics, Special Shared Facility, 12502 North Pine Drive, Tampa (Florida 33612, USA), Fax +1 813 975 7127, e-mail: dgrimm@shctampa.usf.edu , US;(2) H. Lee Moffitt Cancer & Research Institute, Molecular Biology Core Facility, 12902 Magnolia Drive, Tampa (Florida 33612, USA), US;(3) University of Minnesota, College of Veterinary Medicine, Department of Veterinary Pathobiology, Room 300C, Veterinary Science, 1971 Commonwealth Avenue, St. Paul (Minnesota 55108, USA), US;(4) Shriners Hospital for Children, Center for Research in Skeletal Development and Pediatric Orthopedics, Chemistry Section, 12502 North Pine Drive, Tampa (Florida 33612, USA), US;(5) University of South Florida, Institute of Biomolecular Science and College of Medicine, Department of Biochemistry and Molecular Biology, Tampa (Florida 33612, USA), US;(6) Shriners Hospital for Children, Center for Research in Skeletal Development and Pediatric Orthopedics, Tissue Repair Section, 12502 North Pine Drive, Tampa (Florida 33612, USA), US |
| |
| Abstract: | Factor V is a plasma protein essential for blood coagulation. This protein is involved in activated protein C resistance,
the most common inherited thrombotic disorder known. We utilized the polymerase chain reaction to clone the porcine factor
V gene by generating overlapping clones amplified with primers chosen by comparison with known nucleotide sequences. The porcine
factor V cDNA contig encodes a predicted 2258-amino acid protein, making it the largest in comparison to the bovine, human,
and murine proteins. Porcine factor V has the highest level of homology with bovine factor V, but also has high levels of
conservation of important residues with all the species. Radiation hybrid mapping assigned the porcine factor V gene to chromosome
4. Three-dimensional models of factor V were generated and used to analyze membrane-binding sites in terms of conserved, and
therefore likely important residues.
Received 3 October 2000; revised 23 November 2000; accepted 6 December 2000 |
| |
| Keywords: | , Factor V, cloning, gene mapping, modeling, comparative anatomy, porcine, |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
