| 毛细管区带电泳测定八角中莽草酸含量 |
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| 引用本文： | 罗英,罗娅君,何登良,黄礼丽,刘家琴.毛细管区带电泳测定八角中莽草酸含量[J].兰州大学学报(自然科学版),2008,44(6). |
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| 作者姓名： | 罗英 罗娅君 何登良 黄礼丽 刘家琴 |
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| 作者单位： | 绵阳师范学院化学与化学工程系,四川,绵阳,621000 |
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| 基金项目： | Supported by the Administration Department of Education,Sichuan Province(2006A142). |
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| 摘 要： | 建立了八角中莽草酸的毛细管区带电泳-紫外检测分析新方法．在单一磷酸盐和硼砂溶液中由于电扩散现象，莽草酸峰宽并严重前倾或脱尾，采用磷酸盐-硼砂混合溶液为缓冲液改善了峰形并提高了检测灵敏度，通过优化获得其最佳分离条件为pH8．63，10mmol／L NaH2PO4-9mmol／L硼砂，分离电压25kV．该条件下莽草酸的峰面积与浓度呈良好的线性关系，线性方程的相关系数为0．9998，检测限（S／N=3）为0．12μg／mL，比文献报道的方法约高10倍．该方法成功用于八角壳和籽中莽草酸的定量分析，其加标回收率分别为98．9％和104．6％．
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| 关 键 词： | 毛细管区带电泳 八角 莽草酸 |
Determination of shikimic acid in Illicium verum Hook F.by capillary zone electrophoresis |
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| LUO Ying,LUO Ya-jun,HE Deng-liang,HUANG Li-li,LIU Jia-qin.Determination of shikimic acid in Illicium verum Hook F.by capillary zone electrophoresis[J].Journal of Lanzhou University(Natural Science),2008,44(6). |
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| Authors: | LUO Ying LUO Ya-jun HE Deng-liang HUANG Li-li LIU Jia-qin |
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| Abstract: | A simple and rapid capillary zone electrophoresis method using phosphate-borate mixture as running electrolyte with direct UV detection was developed for the analysis of shikimic acid.In pure phosphate and borate buffer,electro-dispersion of sample zone leads to peak leading and tailing because the motilities have the relationship as borate>shikimic acid>phosphate.Using a mixture of phosphate and borate as buffer,the peak efficiency and adsorption signal of shikimic acid are much improved.Af- ter optimizati... |
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| Keywords: | capillary zone electrophoresis Illicium verum Hook F shikimic acid |
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