| 耐有机溶剂脂肪酶产生菌的筛选和鉴定 |
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| 引用本文： | 彭仁,林金萍,魏东芝.耐有机溶剂脂肪酶产生菌的筛选和鉴定[J].江西师范大学学报(自然科学版),2011,35(2):197-200. |
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| 作者姓名： | 彭仁 林金萍 魏东芝 |
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| 作者单位： | 1. 华东理工大学反应器工程国家重点实验室,上海,200237;江西师范大学生命科学学院,江西,南昌,330022
2. 华东理工大学反应器工程国家重点实验室,上海,200237 |
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| 基金项目： | 国家重点基础研究发展计划，江西省科技支撑计划 |
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| 摘 要： | 从国内各地采取土样分离筛选产耐有机溶剂脂肪酶的新菌株,通过形态和生理生化特征以及系统进化树分析,筛选到的新菌株命名为Pseudomonas aeruginosa CS-2.来自Pseudomonas aeruginosa CS-2的粗脂肪酶液在乙腈中酶活提高,在苯、氯仿、正己烷、石油醚和异辛烷中表现出较高的稳定性.
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| 关 键 词： | 铜绿假单胞菌 有机溶剂耐受性 脂肪酶 |
The Screening and Identification of a Novel Strain Producing an Organic Solvent-Tolerant Lipase |
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| PENG Ren,LIN Jin-ping,WEI Dong-zhi.The Screening and Identification of a Novel Strain Producing an Organic Solvent-Tolerant Lipase[J].Journal of Jiangxi Normal University (Natural Sciences Edition),2011,35(2):197-200. |
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| Authors: | PENG Ren LIN Jin-ping WEI Dong-zhi |
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| Institution: | PENG Ren 1,2,LIN Jin-ping1,WEI Dong-zhi1*(1.State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China,2.College of Life Science,Jiangxi Normal University,Nanchang Jiangxi 330022,China) |
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| Abstract: | A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China.The strain was identified as Pseudomonas aeruginosa CS-2 by morphological,physiological and biochemical features and phylogenetic tree analysis.The crude lipase was activated in the presence of acetonitrile and it exhibited high stability in presence of benzene,chloroform,n-Hexane,petroleum ether and isooctane. |
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| Keywords: | Pseudomonas aeruginosa organic solvents tolerance lipase |
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