Absence of low molecular weight DNA polymerase activity from the nuclei ofAmoeba discoides |
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Authors: | G F Abbott Shirley E Hawkins |
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Institution: | (1) Department of Zoology, King's College, Strand, WC2R 2LS London, (England) |
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Abstract: | Summary
Amoeba discoides nuclear protein partially purified by passage through Sephadex G-200 showed 3 high-mol.-wt DNA polymerase activities which eluted in and just following the void volume. No low-mol.-wt (45,000 daltons) DNA polymerase activity was detected. Nuclear protein layered on 5–20% sucrose gradients also showed an absence of lowmol.-wt DNA polymerase . The void volume enzyme showed deoxyribonuclease activity, but no low-mol.-wt nuclease activity was detected. |
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