| 花椰菜花药培养试验研究 |
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| 引用本文： | 张绪璋,周元昌.花椰菜花药培养试验研究[J].河南科技大学学报(自然科学版),2006,27(4):69-71. |
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| 作者姓名： | 张绪璋 周元昌 |
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| 作者单位： | 福建农林大学,作物科学学院,福建,福州,350002 |
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| 基金项目： | 福建省青年科技创新基金(2001j048) |
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| 摘 要： | 以杂交品种B-21为材料,对花椰菜的花药培养条件进行了分析。试验结果表明:花药消毒处理采用0.1%升汞消毒30min效果较好,成活率达86.67%;E3培养基愈伤组织诱导率为86.11%,比MS培养基提高4.40%;用培养基MS 0.5 mg/L6-BA 0.5 mg/L NAA出芽率为31.67%,分化绿苗率为94.79%;用生根培养基1/2 MS 0.1 mg/L NAA 活性碳2 g/L生根率达100%。
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| 关 键 词： | 花椰菜 花药培养 愈伤组织诱导率 分化绿苗率 生根率 |
| 文章编号： | 1672-6871（2006）04-0069-03 |
| 收稿时间： | 2006-01-15 |
| 修稿时间： | 2006年1月15日 |
Experimental Studies on the Anther Culture of Brassica Oleracea Var. Botrytis |
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| ZHANG Xu-Zhang,ZHOU Yuan-Chang.Experimental Studies on the Anther Culture of Brassica Oleracea Var. Botrytis[J].Journal of Henan University of Science & Technology:Natural Science,2006,27(4):69-71. |
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| Authors: | ZHANG Xu-Zhang ZHOU Yuan-Chang |
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| Abstract: | Conditions for the anther culture of brassica oleracea var. botrytis was studied using hybrid variety B-21 as material.Results show that sterilization with 0.1% HgCl_2 for 30mins is the best treatment,resulting in a survival rate up to 86.67%.The rate of callus induction on E3 medium is 86.11%,4.40% higher than that on MS medium.The rate of plantlet differentiation on MS medium plus 0.5mg/L NAA is 31.67%.100% of root differentiation is obtained on 1/2MS medium plus 01mg/L NAA and 2 g/L active carbon. |
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| Keywords: | Brassica oleracea var botrytis Anther culture Rate of callus inducement Rate of plantlet differentiation Rate of root differentiation |
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