间歇出酶对里氏木霉产纤维素酶的影响

在分批补料的基础上,采用间歇出酶的方法,对里氏木霉产纤维素酶进行了研究。结果表明:分批补料过程中最佳的补料速度为5.5 g/(L·d),在此条件下产酶第8天滤纸酶活力和β-葡萄糖苷酶活力达到14.40 U/mL和1.59 U/mL。在分批补料的基础上进行间歇出酶,与对照相比,第4、6、8天出酶模式(模式1)时,总滤纸酶活力提高18.14%; 第4、7天出酶模式(模式2)时,总滤纸酶活力提高17.75%; 第5、8天出酶模式(模式3)时,总滤纸酶活力提高27.35%。研究表明,通过间歇出酶可以有效提高纤维素酶总滤纸酶的活力。

Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei

This paper investigated fed-batch and periodic harvest of enzyme for cellulase production by Trichoderma reesei. The results showed that the best feeding rate was 5.5 g/(L·d)cellulose per day, and the filter paper activity and β-glucosidase were 14.40 U/mL and 1.59 U/mL after 8 days of cultivation. Based on fed-batch, when harvesting enzyme at the 4^th, 6^th, 8^th day, the total filter paper activity improved by 18.14%. When harvesting at the 4^th,7^th day, the total filter paper activity improved by 17.75%. When harvesting at the 5^th,8^th day, the total filter paper activity improved by 27.35%.This paper concluded that periodic harvest of enzyme could improve the total filter paper activity efficiently.