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| 热激和热胁迫过程中玉米幼苗谷胱甘肽还原酶活性和同工酶的变化 | |
| 引用本文： | 郭丽红,陈善娜,王德斌,龚明.热激和热胁迫过程中玉米幼苗谷胱甘肽还原酶活性和同工酶的变化[J].云南大学学报(自然科学版),2006,28(3):262-266. |
| 作者姓名： | 郭丽红 陈善娜 王德斌 龚明 |
| 作者单位： | 1. 昆明师范高等专科学校,生物系,云南,昆明,650031 2. 云南大学,生命科学学院,云南,昆明,650091 3. 云南师范大学,生命科学学院,云南,昆明,650092 |
| 基金项目： | 国家自然科学基金资助项目(39660009)，“211工程”二期建设费及植物学博士点经费资助项目，昆明师范高等专科学校重点学科建设基金资助项目(Z-002) |
| 摘 要： | 以玉米品种晴3和大黄为材料,研究结果表明热激幼苗的存活率比未热激幼苗高,这说明热激提高了玉米幼苗的抗热性.热激幼苗的谷胱甘肽还原酶活性明显高于未经热激的幼苗,同时热激处理后,GR的同工酶谱在整个热胁迫期间与对照相比也存在差异,说明热激过程中谷胱甘肽还原酶活性的升高和同工酶的变化与玉米幼苗的抗热性相关. |
| 关 键 词： | 热激 玉米幼苗 谷胱甘肽还原酶 GR同工酶 |
| 文章编号： | 0258-7971(2006)03-0262-05 |
| 修稿时间： | 2005年11月25 |
Changes in activity of glutathione reductase and it's isozyme of maize seedlings during heat shock and heat stress |
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| GUO Li-Hong,CHEN Shan-na,WANG De-bin,GONG Ming.Changes in activity of glutathione reductase and it''''s isozyme of maize seedlings during heat shock and heat stress[J].Journal of Yunnan University(Natural Sciences),2006,28(3):262-266. | |
| Authors: | GUO Li-Hong CHEN Shan-na WANG De-bin GONG Ming |
| Institution: | 1. Department of Biology, Kunming Teachers College, Kunming 650031, China;2. School of Life Sciences, Yunnan University, Kunming 650091, China;3. Department of Life Sciences, Yunnan Normal University, Kunming 650092, China |
| Abstract: | Two varieties of maize(Zea maysL.,cultivars Dahuang and Qing No.3) were used in the study.The results showed that a heat shock(HS) treatment significantly increased the survival percentage of two variety seedlings at heat stress,confirming heat-shock increased heat tolerance of maize seedlings.More-over,the maize seedlings through heat pretreatment,retained relativelyhigher activity of glutathine reductase(GR,EC 1.6.4.2) than the seedlings without heat-shock pretreatment.In the profiles glutathine reductase isozyme in the seedlings after heat-shock-pretreatment appeared a new band as compared with the seedlings without heat-shock pretreatment.Changes in isozyme profiles of GR had been different during heat stress.These indicated thathigher activity of GR and changes in isozyme profiles of GR were related to heat tolerance of maize seedlings. |
| Keywords: | heat shock maize seedling glutathine reductase glutathine reductase isozyme |
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