| 厚荚相思组织培养与快速繁殖 |
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| 引用本文： | 赖家业,周传明,叶春生,黄寿先,韦鹏霄,岑秀芬,陈放. 厚荚相思组织培养与快速繁殖[J]. 四川大学学报(自然科学版), 2003, 40(5): 982-985 |
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| 作者姓名： | 赖家业 周传明 叶春生 黄寿先 韦鹏霄 岑秀芬 陈放 |
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| 作者单位： | 四川大学生命科学学院,成都,610064;广西大学林学院,南宁,530001;广西大学林学院,南宁,530001;广西区林业局种子站,南宁,530022;广西大学生物技术实验中心,南宁,530005;四川大学生命科学学院,成都,610064 |
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| 基金项目： | 广西科学基金(桂科基0342013),广西林业科技项目(林科字[2000]第86号) |
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| 摘 要： | 以5年生厚荚相思(Acacia crassicarpa)的萌芽茎段为外植体进行了组织培养的初步研究,结果表明：改良的MS 6-BA2．0mg／L NAA0．2mg／L培养基能够较好地诱导芽的分化和增殖;适宜的继代增殖培养基为改良MS 6-BA1．5mg／L NAA0．2mg／L;较为想的生根培养基为1／2MS IBA0．5mg／L,生根率达95．0％;生根苗的移栽基质以黄心土的移栽效果最好,移栽成活率达90．0％。
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| 关 键 词： | 厚荚相思 快速繁殖 植株再生 |
| 文章编号： | 0490-6756(2003)05-0982-04 |
In Vitro Micropropagation of Acacia crassicarpa |
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| LAI Jia ye ,,ZHOU Chuan ming ,YE Chun sheng ,HUAN Shou xian ,WEI Peng xiao ,CHEN Xiu fen ,CHEN Fang. In Vitro Micropropagation of Acacia crassicarpa[J]. Journal of Sichuan University (Natural Science Edition), 2003, 40(5): 982-985 |
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| Authors: | LAI Jia ye ZHOU Chuan ming YE Chun sheng HUAN Shou xian WEI Peng xiao CHEN Xiu fen CHEN Fang |
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| Affiliation: | LAI Jia ye 1,2,ZHOU Chuan ming 2,YE Chun sheng 3,HUAN Shou xian 2,WEI Peng xiao 4,CHEN Xiu fen 4,CHEN Fang 1 |
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| Abstract: | A tissue culture method is described for clonal multiplication of Acacia crassicarpa using nodal explants from the trees growing in the field. Results showed that the improved MS medium with 2.0 mg/L 6 BA and 0.2mg/L NAA was the optimum medium for induction and proliferation of bud clusters. The suitable culture medium for the generation continuing multiplication is the improved MS supplemented with 1.5mg/L 6 BA and NAA 0.2mg/L.The shoots rooted best on 1/2MS medium supplemented with 0.5 mg/L IBA. Plantlet survival after transfer to soil was 90.0%. |
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| Keywords: | Acacia crassicarpa micropropagation plant regeneration |
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