| 烟草和元麦叶肉原生质体吸收尼古丁机制的研究 |
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| 引用本文： | 黄祥辉,虞乃而,颜季琼.烟草和元麦叶肉原生质体吸收尼古丁机制的研究[J].上海师范大学学报(自然科学版),1992(1). |
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| 作者姓名： | 黄祥辉 虞乃而 颜季琼 |
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| 作者单位： | 华东师范大学生物学系，华东师范大学生物学系，华东师范大学生物学系 |
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| 摘 要： | 用EA_3-867纤维素酶分离的烟草(Nicotianatabacum)和元麦(Hordeumdistichon)叶肉原生质体,分别保温在尼古丁溶液中,用紫外分光光度计测定它们对尼古丁的吸收。结果表明,这两种原生质体能迅速地吸收尼古丁;原生质体吸收尼古丁的量,随介质尼古丁浓度的升高而增多;在生理学pH范围内,介质的pH对原生质体吸收尼古丁没有多大的影响。初步认为,这两种原生质体对尼古丁的吸收是一种简单的扩散机制。
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| 关 键 词： | 尼古丁 扩散 原生质体 吸收 烟草 元麦 |
Study on Mechanism of Nicotine Uptake by Nicotiana tabacum and Hordeum distichon Mesophyll Protoplasts |
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| HUANG XIANGHUI YU NAIER YAN JIQIONG YEN CHI CHIONG.Study on Mechanism of Nicotine Uptake by Nicotiana tabacum and Hordeum distichon Mesophyll Protoplasts[J].Journal of Shanghai Normal University(Natural Sciences),1992(1). |
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| Authors: | HUANG XIANGHUI YU NAIER YAN JIQIONG YEN CHI CHIONG |
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| Institution: | HUANG XIANGHUI YU NAIER YAN JIQIONG YEN CHI CHIONG Department of Biology |
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| Abstract: | Mesophyll protoplasms of the Nicotiana tabacum and Hordeum distichon released by EA_3-867 cellulase,respectively were incubated in medium containing nicotine. Nicotine uptake by the protoplasts was measured with Beckman DU-7 spectropho- tometer.The results indicates that the nicotine was rapidly uptaked by the two species protoplasts;The quantity of the nicotine uptake by the protoplasms increased along with raise of the nicotine concentration;In physiological pH range,the effect of pH on the nicotine uptake by the protoplass was small.In light of the abo- ve findings,the preliminary assumption is that the nicotine uptake mechanism by two species protoplasts is a simple diffusion. |
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| Keywords: | nicotine diffunsion protoplasts uptake Nicotiana tabacum Hordeum distichon |
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