| 乙醇酸氧化酶和模式生物含有富苯丙氨酸的碱性蛋白 |
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| 引用本文： | 曾广翔,陈佳,陈雪敏,黄颖沂,谢莉,林子衍,陈梓谦,徐杰.乙醇酸氧化酶和模式生物含有富苯丙氨酸的碱性蛋白[J].华南师范大学学报(自然科学版),2010,0(4). |
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| 作者姓名： | 曾广翔 陈佳 陈雪敏 黄颖沂 谢莉 林子衍 陈梓谦 徐杰 |
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| 作者单位： | 1.1.2.华南示范大学生命科学学院 |
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| 基金项目： | 广东省高等教育本科教学改革项目 |
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| 摘 要： | 作者在之前研究中已证实提取的乙醇酸氧化酶(GO)实际上是GO和富phe碱性蛋白(BP)的混合物,BP因在SDS-PAGE中向负极泳动而容易被忽略.该文再次提取比活为113.4 U/(min.mg)的GO和BP的混合物.该混合物呈现phe的258 nm特征吸收峰,表明BP可能位于GO外部.类似的BP也可能存在于拟南芥、水稻等模式植物和大肠杆菌的粗蛋白中.
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| 关 键 词： | 乙醇酸氧化酶（GO） 富phe碱性蛋白（BP） SDS-电泳 负极泳动 |
| 收稿时间： | 2009-11-10 |
PHE-RICH BASIC PROTEIN EXISTED IN GLYCOLATE OXIDASE AND IN SEVERAL MODEL ORGANISMS |
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| ZENG Guangxiang,CHEN Jia,CHEN Xuemin,HUANG Yingyi,XIE Li,LIN Ziyan,CHEN Zhiqian,XU Jie.PHE-RICH BASIC PROTEIN EXISTED IN GLYCOLATE OXIDASE AND IN SEVERAL MODEL ORGANISMS[J].Journal of South China Normal University(Natural Science Edition),2010,0(4). |
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| Authors: | ZENG Guangxiang CHEN Jia CHEN Xuemin HUANG Yingyi XIE Li LIN Ziyan CHEN Zhiqian XU Jie |
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| Abstract: | Abstract Glycolate oxidase (GO) is a protein composed of identical subunits of 40 kDa, but often exhibits different pI values. In previous study, we had proved that extracted GO was actually a mixture GO and phe-rich basic protein (BP), and the BP was ignored easily as it migrated towards the cathode in SDS-electrophoresis. In this paper, the mixture of GO and BP with a specific activity of 121 U/min.mg was extracted. The UV spectrum of the mixture showed an absorption peak at 258 nm which is the characteristic adsorption of phenylalanine, suggesting that BP located on the surface of GO. Similar BP also existed in crude protein extracts from several mode species of organism, including Arabidopsis thaliana, Oryza sativa and Escherichia Coli. |
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