大鼠在空间辨别性学习记忆时海马CA1,CA3区和DG的ACh能纤维和星形胶质细胞的变化

目的通过建立大鼠空间辨别性学习记忆的动物模型,观察大鼠海马CA1,CA3区和齿状回(DG)的乙酰胆碱(ACh)能纤维密度和含量的变化以及星形胶质细胞的数量及其胶质原纤维酸性蛋白(GFAP)表达的变化,从形态学上探讨中枢ACh和星形胶质细胞与空间辨别性学习记忆的关系.方法建立空间辨别性学习记忆的动物模型,采用乙酰胆碱酯酶(AChE)的组织化学染色及GFAP标记星形胶质细胞.并用显微镜方格目测系统和图像分析仪对大鼠海马CA1,CA3区及DG的GFAP免疫阳性星形胶质细胞的数量及其GFAP表达进行检测.结果模型组与对照组相比大鼠各观察部位的AChE阳性纤维的密度和含量均增高,有显著性差异(P<0.05).而空白组和游水组相比大鼠各观察部位的AChE阳性纤维的密度和含量均无显著性差异(P>0.05),模型组内各组间两两比较大鼠各观察部位的AChE阳性纤维的密度和含量均无显著性差异(P>0.05);模型组与对照组相比大鼠各观察部位的星形胶质细胞的数量及其GFAP的表达均增加,有显著性差异(P<0.05).而空白组和游水组相比大鼠各观察部位的星形胶质细胞的数量及其GFAP的表达均无显著性差异(P>0.05),模型组内各组间两两比较大鼠各观察部位的星形胶质细胞的数量及其GFAP的表达均无显著性差异(P>0.05).结论在空间辨别性学习记忆过程中,大鼠各观察部位的AChE阳性纤维的密度和含量增高,说明中枢ACh参与空间辨别性学习记忆过程;在空间辨别性学习记忆过程中,大鼠各观察部位的星形胶质细胞的数量及其GFAP表达的增加,说明星形胶质细胞参与空间辨别性学习记忆过程.

The Change of Acetylcholine Fibers and Astrocytes in Hippocampal CA1,CA3 and Dentate Gyrus when Spatial Differentiating Learning and Memory in Rats

Objective To explore the roles of acetylcholinergic fibers and astrocytes in differentiating spatial learning and memory in rats,we observed the content of acetylcholinergic fibers and numbers of astrocytes together with the expression of GFAP in hippocampal CA1,CA3 and DG by establishing differentiating spatial learning and memory animal models in water maze.Methods Through Establishing differentiating spatial learning and memory animal models,we carried through the histochemical staining and determination of acetylcholinergic fibers and marked GFAP positive astrocytes in hippocampal CA1,CA3 and DG in rats.We made statistical analysis on GFAP positive astrocytes and the expression of GFAP through microscope checked visualization system and image analysis apparatus in hippocampal CA1,CA3 and DG.Results The spatial learning and memory models were successfully established through 7 days training in water maze.The AChE positive fiber density and content in CA1,CA3 and DG in model groups was higher than that of controlled group(P<0.05).But the AChE positive fiber density in swimming group was not obvious difference compared with that of normal controlled group(P>(0.05)).There was not obvious difference of AChE positive fiber density and content among model groups(P>(0.05)).The quantity of astrocytes and the expression of GFAP in CA1,CA3 and DG in model groups were higher than that of controlled group(P<(0.05)).But the quantity of astrocytes and the expression of GFAP between normal controlled group and swimming group have not obvious difference(P>(0.05)).There was not obvious difference of quantity of astrocytes and expression of GFAP among model groups(P>(0.05)).Conclusion In the spatial learning and memory animal models,the AChE positive fiber density and content in hippocampal CA1,CA3 and DG increased.This demonstrated that ACh was concerned with learning and memory process.The number and GFAP expression in astrocytes were enhanced in spatial learning and memory process,this also proved that astrocytes took a crucial role in spatial learning and memory.