竹叶多糖的提取工艺

目的：筛选竹叶多糖提取优化的最佳工艺.方法：以竹叶多糖为工艺筛选指标,采用正交实验设计方法筛选最佳提取工艺及其条件.结果：竹叶多糖最佳提取工艺为石油醚回流2 h，滤渣用95%乙醇回流2 h，再用30倍量的水回流3次浸提多糖.用Sevage法除蛋白,加入乙醇使含醇量为80%,于冰箱静置12 h,得多糖沉淀.采用优化后的工艺提取竹叶多糖粗品的得率约0.51%.结论：采用该提取工艺,竹叶多糖的提取率较高.

Extraction Process of Polysaccharides in Bamboo Leaves

ObjectiveTo optimize the best extraction process of polysaccharides in bamboo leaves.MethodsWith polysaccharides of bamboo leaves being an examinable target component，the optimized extraction techniques and condition were chosen by orthogonal test method.ResultsThe best extraction process of polysaccharides of bamboo leaves was that the crude drugs were extracted with petroleum ether for refluxed 2 h，the sediments extracted with 95% ethanol for 2 h first，then filtrable residue from previous steps were extracted three times with 30 times amount of water.The proteins were removed by Sevage’s method，adding ethanol and making its concentration to 80%，the extracts were stored in the refrigerator for 12 h.The acquired amount of bamboo leaves polysaccharides was about 0.51% from the best extraction process.Conclusion:The extractive rate of bamboo leaves polysaccharides was higher in the optimized extraction process and the process was proved to be reasonable and feasible.