| 芥菜茎中水溶性多糖的分离纯化及性质的初步研究 |
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| 引用本文： | 李夏兰,魏国栋,王昭晶,陈培钦,蔡婀娜. 芥菜茎中水溶性多糖的分离纯化及性质的初步研究[J]. 福建师范大学学报(自然科学版), 2006, 22(2): 72-75 |
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| 作者姓名： | 李夏兰 魏国栋 王昭晶 陈培钦 蔡婀娜 |
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| 作者单位： | 1. 华侨大学生物工程系,福建,泉州,362011;华侨大学制药工程研究所,福建,泉州,362011
2. 华侨大学生物工程系,福建,泉州,362011 |
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| 基金项目： | 华侨大学校科研和教改项目;国务院侨务办公室科研项目 |
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| 摘 要： | 采用水提醇沉法从芥菜中提取出水溶性粗多糖CP,经Sevag法脱蛋白和H2O2去色素,过DEAE-纤维素柱,经水洗涤和盐洗脱得到BPA,再经Sephadex G-100凝胶层析纯化得BPA 1.采用SephadexG-150凝胶过滤层析和醋酸纤维纸电泳鉴定,初步鉴定BPA 1为纯品.红外光谱检测证明BPA 1是多糖.
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| 关 键 词： | 芥菜 多糖 分离 鉴定 |
| 文章编号： | 1000-5277（2006）02-0072-04 |
| 收稿时间： | 2005-08-26 |
| 修稿时间： | 2005-08-26 |
Isolation,Purification,Character Research of Polysaccharide from the Stalk of Brassica juncea |
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| LI Xia-lan,WEI Guo-dong,WANG Zhao-jing,CHEN Pei-qing,CAI E-na. Isolation,Purification,Character Research of Polysaccharide from the Stalk of Brassica juncea[J]. Journal of Fujian Teachers University(Natural Science), 2006, 22(2): 72-75 |
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| Authors: | LI Xia-lan WEI Guo-dong WANG Zhao-jing CHEN Pei-qing CAI E-na |
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| Affiliation: | 1. Department of Bioengineering, Huaqiao University, Quanzhou 362011, China; 2. Research Institute of Pharmaceutical Engineering, Huaqiao University, Quanzhou 362011, China |
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| Abstract: | The water-soluble crude polysaccharide CP were obtained from the stalk of Brassica juncea by boiling-water extraction and ethanol precipitation.After the proteinic polysaccharides was removed by Savage way and the pigment in polysaccharides was removed by H_2O_2,CP was fractionated by DEAE-cellulose column chromatography,giving one polysaccharide fractions termed BPA.It was further purified with Sephadex-100 column chromatography,BPA1 was obtained.The Sephadex-150 gel filtration chromatography and cellulose acetate pellicle electrophoresis proved that BPA1 were purematerial. |
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| Keywords: | Brassica juncea polysaccharides isolation identify |
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