新型气相毛细管柱的制备及其色谱分离

将羟丙基--环糊精(HP--CD)静态涂覆在毛细管内壁得到一种新的气相毛细管柱，以正十二烷为溶质测得该色谱柱的理论塔板数为2337/m，与涂覆七-(2,6-O-二戊基-3- O-三氟乙酰基)--环糊精(DP-TFA--CD)的毛细管柱柱效相近；在这两种毛细管柱上对对5种二取代苯系化合物(如硝基甲苯、硝基氯苯、二氯苯、二甲苯和苯二酸二甲酯)的位置异构体进行分离研究。结果表明，它们对邻位/对位、间位/对位取代苯系化合物表现较好的分离能力，在HP--CD柱上，二氯苯异构体的分离因子分别为1.28和1.13，比DP-TFA--CD柱的分离效果好。

Preparation of New Gas Phase Capillary Columns Coated withβ-Cyclodextrin Derivatives and Separation Behaviors toward Benzene Positional Isomers

A new capillary column was prepared by statically coating hydroxypropyl--cyclodextrin (HP--CD) onto inner surface of fused capillary and its theoretical plate number is measured to be 2337 /m according to the literature method, which is close to a commercial gas phase capillary column coated with heptakis-(2, 6-O-dipentyl-3-O-trifluoro acetyl)--cyclodextrin (denoted as DP-TFA--CD). Separations of some benzene positional isomers, namely, nitrotoluene, nitrochlorobenzen, dichlorobenzene, xylene, and dimethyl phthalate, were investigated on these two capillary columns in gas chromatography. The HP--CD and DP-TFA--CD columns show good separation abilities toward five selected analytes. Separation factors for dichlorobenzene on the HP--CD column are 1.28 and 1.13, respectively, which are better than the other.