耐力训练对大鼠骨骼肌收缩蛋白的影响

目的:探索运动训练对大鼠骨骼肌收缩蛋白的影响,为进一步研究其蛋白水平提供依据。方法:选用清洁级健康雄性Sprague-Dawley(SD)大鼠20只,6周龄,体重135-150g,随机分为2组。A:安静对照组,n=10,B:运动训练组,n=10。运动训练组:进行持续性大运动量跑台训练8周。实验使用SDS-PAGE(十二烷基硫酸钠-聚丙烯凝胶电泳),浓缩胶浓度5%,分离胶浓度10%,电泳电压160V,时间200min。结果:在骨骼肌收缩蛋白SDS-PAGE后,运动训练组电泳图谱上出现大量新的条带,分子量范围为43-200 kDa。结论:本运动模型下,在骨骼肌收缩蛋白SDS-PAGE后,运动训练组图谱上出现大量新的蛋白条带,这些新的条带是肌肉蛋白质的降解产物,还是为了修复运动损伤而合成的新的蛋白质片段,还有待后续研究。

Endurance Training on the Contraction Proteins in Rat Skeletal Muscle

Influences of exercise training on the contraction proteins in rat skeletal muscle.objective: To explore the influences of exercise training on the contraction proteins and the gene expression of myosin heavy chain(MHC) isoforms in rat skeletal muscle,and to supply some basis for next research about the mechanism.Method: To select 20 clean and healthy male Sprague-Dawley(SD),which were 6 weeks ages and weighted from 135g to 150g,the paper divided them into 2 groups stochastically: A、n=10,B、HT,n=10.Heavy-load training Group carried on the long-enduring and great-load running exercise for 8 weeks.SDS-PAGE was adapt as follow: Stacking gel was kept under the density of 5%,and separating gel under the density of 10%.Electrophoresis voltage was 160 V for200 minutes.Results: After the skeletal muscle contraction protein took the SDS-PAGE,a largenumber of new strips appeared in the collection of illustrative plates in HT group.The range of molecular weight 43-200 kDa.Conclusion: On this exercise model,a large number of new protein strips appeared in the collection of illustrative plates in HT group after the skeletal muscle contraction protein took the SDS-PAGE.It is not clear whether they are degraded fragments or new anabolic ones for the next research.