西归挥发油抗痛经作用研究

目的研究西归挥发油对痛经的影响,初步探讨其抗痛经作用。方法制备离体子宫标本,通过BL-420F生物机能系统观察西归挥发油对正常离体子宫自发活动及其对缩宫素诱发子宫收缩的影响。同时建立大鼠痛经模型和小鼠寒凝血瘀型痛经模型,观察西归挥发油的镇痛作用,并观察子宫、卵巢出现的病理变化,评价西归挥发油的抗痛经作用。结果离体实验中,西归挥发油各浓度均能不同程度使正常离体子宫自发性收缩和缩宫素诱发收缩的频率、振幅、峰面积降低,持续时间升高(P 0.05或P 0.01),且明显的剂量依赖性。其中西归挥发油0.2、0.1、0.05 g/mL剂量组抑制作用显著(P 0.01)。在体实验中,西归挥发油高、中、低剂量组对大鼠痛经模型的子宫平滑肌痉挛有抑制作用,可使潜伏期延长,与模型组相比有显著性差异(P 0.05或P 0.01),且西归油中、低剂量组可显著减少扭体次数(P 0. 05或P 0.01)。但西归挥发油对小鼠血瘀型痛经作用不明显。结论西归挥发油抗痛经作用,其作用可能与调节子宫平滑肌的兴奋性有关。

ANTI-DYSMENORRHEA EFFECTS OF XIGUI ESSENTIAL OIL

Objective:To study the anti-dysmenorrhea effects and corresponding mechanism of Xigui essential oil. Methods:After preparation of isolated uterus specimens in vitro, the effects of Xigui essential oil on the spontaneous activity of normal isolated uterus and its inhibition effects on uterine contraction induced by oxytocin were observed by BL-420F biological function system. Meanwhile, the models of dysmenorrhea in rat and cold clotting dysmenorrhea in mice were established, and the analgesic effects of Xigui essential oil were observed. The pathological changes of the uterus and ovaries in these animals were also evaluated to explore the anti-dysmenorrhea effect of essential oil. Results:In vitro, the Xigui essential oil at different concentrations reduced the spontaneous activity of normal isolated uterus, the frequency, amplitude and peak area of uterine contraction induced by oxytocin, and increased the duration (P<0.05 or P<0.01). And these effects were significantly dose-dependent, and were highly significant at the concentrations of 0.2, 0.1, 0.05 g/mL (P<0.01). In vivo, the high, medium and low doses of the Xigui essential oil inhibited the spasm of uterine smooth muscle in rat''s dysmenorrhea model, significantly increased the latency period compared to the model group (P<0.05 or P<0.01). The middle and low dose groups of the essential oil significantly reduced the number of writhing in mice (P<0.05 or P<0.01). However, the Xigui essential oil from had no obvious anti-dysmenorrhea effect. Conclusion:The Xigui essential oil has anti-dysmenorrhea effect, the mechanism are related to the excitability of uterine smooth muscle.