腐浆中细菌的鉴定及其生物膜活性研究

纸机运转过程中的腐浆障碍主要是由系统内微生物所引起的。从UPM常熟纸厂PM2纸机机架的腐浆里筛选得到10株细菌N1、N2……N10,通过对其形态特征、生理生化特性以及16SrRNA序列分析,初步鉴定N1、N3为鞘氨醇单孢菌(Sphingomonas azotifigens)、N2为腐败假单孢菌(Pseudomonas putrefaciens)、N4为未培养细菌(uncultured bacterium)、N5为Filimonas lacunae、N6为Asticcacaulis excentricus、N7为少动假单孢菌(Pseudomonas paucimobilis)、N8为Siphonobacteraquaeclarae、N9为不解糖鞘氨醇单孢菌(Sphingomonas pruni)、N10为Flectobacillus Larkin。将分离后的10株细菌分别回接到含细小纤维的回接试样中,检测腐浆细菌的生物膜对细小纤维的絮聚情况,结果显示N5、N8、N10对细小纤维有很大的絮凝沉淀作用,由此推断这3株细菌的生物膜活性强,是腐浆中的有害细菌。

Identification of bacterias from the slime and the study on their bioflim activity

The slime pulp,causing much trouble for the pulp and paper making industry,is usually produced by bacterium.Ten kinds of bacteria including N1,N2,…,N10 were screened from the slime hanging on the PM2 paper machine at UPM.They were identified as Sphingomonas azotifigens(N1,N3),Pseudomonas putrefaciens(N2),uncultured bacterium(N4),Filimonas lacunae(N5),Asticcacaulis excentricus(N6),Pseudomonas paucimobilis(N7),Siphonobacter aquaeclarae(N8),Sphingomonas pruni(N9),Flectobacillus Larkin(N10) through the morphological observation,physiological & chemical tests and 16S rRNA sequence analysis.Inoculate these bacteria to the simulative white water system and detect whether the slime was produced.It was proved that N5,N8,N10 were the key bacteria for the slime.