| 聚乙二醇6000-甲硫氨酸螯合铜修饰物混合吐温80-硫酸铵盐-液-固体系提取血红蛋白 |
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| 引用本文： | 刘波,余学红,靳光才,陈丽娟,沈静茹.聚乙二醇6000-甲硫氨酸螯合铜修饰物混合吐温80-硫酸铵盐-液-固体系提取血红蛋白[J].中南民族大学学报(自然科学版),2008,27(4). |
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| 作者姓名： | 刘波 余学红 靳光才 陈丽娟 沈静茹 |
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| 作者单位： | 1. 中南民族大学,化学与材料科学学院,分析化学国家民委重点实验室,武汉,430074
2. 中南民族大学,校医院,武汉,430074 |
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| 基金项目： | 国家民委重点基金资助项目
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| 摘 要： | 采用聚乙二醇6000修饰物混合吐温80-硫酸铵盐-水液-固体系分离血红蛋白.研究了此萃取体系中修饰物的含量、成相聚合物吐温80的浓度、盐的浓度、体系酸度等因素对血红蛋白在液固两相中分配的影响,并得到最佳分离条件.体系中修饰物含量为2%(质量密度),吐温80含量为4.5%(体积百分数),酸度为pH=7.95,盐浓度为2mol/L.一次固相收得率达到96.5%.
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| 关 键 词： | PEG-Met-Cu修饰物 非有机溶剂液-固萃取体系 血红蛋白 |
Abstraction of Hemoglobin by Mixing Copper-Chelate PEG6000-Methionine Modifier -Tween80- Ammoniuum Sulfate in Liquid-Solid Extraction System |
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| Liu Bo,Yu Xuehong,Jin Guangcai,Chen Lijuan,Shen Jingru.Abstraction of Hemoglobin by Mixing Copper-Chelate PEG6000-Methionine Modifier -Tween80- Ammoniuum Sulfate in Liquid-Solid Extraction System[J].Journal of South-Central Univ for,2008,27(4). |
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| Authors: | Liu Bo Yu Xuehong Jin Guangcai Chen Lijuan Shen Jingru |
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| Institution: | Liu Bo1,Yu Xuehong2,Jin Guangcai1,Chen Lijuan1,Shen Jingru1 |
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| Abstract: | The method of separation and purification of hemoglobin by using inorganic solvent liguid-solid extraction system of PEG-Met-Cu-tween80-(NH4)2SO4 was studied.The distribution of hemoglobin in the liquid-solid two phases can be changed by various factors such as concentrations of modifier,ammonium sulfate,and tween80,the value of pH and so on.Optimum extraction of hemoglobin was achieved at pH 7.95,PEG-Met-Cu modifier 2%(mass density),Tween80 4.5%(volume fraction)(NH4)2SO4 2 mol/L. |
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| Keywords: | PEG-Met-Cu modifier inorganic solvent liquid-solid extraction system hemoglobin |
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