荧光原位杂交在微生物学中的应用及进展

荧光原位杂交技术广泛用于分析复杂环境的微生物群落构成,可以在自然生境中监测和鉴定微生物,并能对未被培养的微生物进行检测.rRNA为靶序列寡核苷酸或PNA探针的荧光原位杂交能提供微生物的形态学、数量、空间分布等信息,现已成为环境科学研究领域中的热点技术.本文对荧光原位杂交技术的发展和在环境微生物学中的应用进行了综述,探讨了该技术应用和发展前景.

APPLICATION AND PROGRESS OF FLUORESCENCE IN SITU HYBRIDIZATION (FISH) IN ENVIRONMENTAL MICROBIOLOGY

Fluorescence In Situ Hybridization(FISH) has been widespread applied for analyzing the composition of microbial communities in complex environmental samples.The examination and identification of individual microbial cell within their natural habitat could be performed,as well as those yet-to-be cultured microorganisms could be visualized by this technology.The information about morphology,number,and spatial distribution of the microorganisms could be provided by FISH with rRNA-targeted oligonucleotides probes or PNA.Furthermore,it is a powerful tool for studying environmental microbiology.In this paper,major techniques and progresses of FISH,and its applications in microbial ecology were reviewed,subsequently the perspectives of FISH were discussed.