天女木兰多酚类物质的季节变化规律

通过对不同月份天女木兰叶中多酚类物质含量的测定,推测天女木兰多酚类物质的季节变化规律;使用高效液相色谱仪定性、定量测定不同月份天女木兰不同高度叶片游离酚、结合酚和酯化酚;采用紫外分光光度法测定多酚的抗氧化性.结果表明:天女木兰叶片含有绿原酸和对香豆酸2种游离态酚;含有咖啡酸、对香豆酸2种结合酚;含有对香豆酸1种酯化酚.5月上旬开始,随着叶的发育天女木兰游离酚的含量逐渐增加,进入7月上旬,达到最高,随后含量逐渐下降;从5月上旬长叶开始至10月下旬落叶为止,总体呈现随着叶的发育酯化酚含量逐渐增加的规律,叶内结合酚呈现出非规律性的变化;5月叶片抗氧化性最弱,5月至8月抗氧化性逐渐上升,8月抗氧化性最高,8月至10月抗氧化性逐渐下降.天女木兰抗氧化性与多酚类物质含量具有相关性,确定天女木兰叶的最佳采集时期为10月上旬,即落叶前期.

Seasonal Variations of Polyphenols of Magnolia sieboldii K. Koch

Through the determination of polyphenols content in different species and the leaves of Magnolia sieboldii,the seasonal variation of M. sieboldii polyphenols was speculated;The free phenol,phenol and phenol of M. sieboldii leaves with different heights in different months were determined qualitatively and quantitatively by using HPLC;Antioxidation of polyphenols was determined by UV spectrophotometry. The results show that the leaves of M. sieboldii contains chlorogenic acid and p-coumaric acid,contains caffeic acid,p-coumaric acid and two kinds of combined phenol;containing a phenol and p-coumaric acid esterification. Beginning in the early May,with the free phenol development of the Magnolia sieboldii leaf increased gradually,reached the highest in the early July, then decreased;from early May to late October until the deciduous leaf, showing an overall development with the content of esterified leaves gradually increased by law;from early May to late October deciduous leaf so far,the leaves of Magnolia sieboldii combined phenol presents the change regularity of the non leaf antioxidant;From May to August,antioxidant activity was the weakest in May,and then gradually increased, was the highest on August, antioxidant activity decreased gradually from August to October. The antioxidant activity and polyphenol content of Magnolia sieboldii has correlation. The best collection period for Magnolia sieboldii is determined in the early October by observing the law.