| 基于DNA指纹鉴定技术的市售川贝母质量考察分析 |
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| 引用本文： | 周亭亭,李明成.基于DNA指纹鉴定技术的市售川贝母质量考察分析[J].北华大学学报(自然科学版),2017,18(6). |
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| 作者姓名： | 周亭亭 李明成 |
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| 作者单位： | 北华大学医学检验学院,吉林吉林,132013;北华大学医学检验学院,吉林吉林,132013 |
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| 基金项目： | 吉林省战略性新兴产业和高新技术产业发展项目，吉林省科技发展计划项目，吉林市科技支撑计划项目，吉林省中医药科技项目 |
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| 摘 要： | 目的 考察当前不同地区市售川贝母的质量情况.方法 应用川贝母PCR检测试剂盒法和药典法对3个城市24批商品川贝母样品进行鉴定.结果 在24批商品川贝母样品中检出18批伪品,6批正品,合格率仅为25%;且试剂盒法与药典法的鉴定结果一致.结论 目前市售川贝母质量状况不容乐观;DNA指纹鉴定方法简单易行,该结果也为我国中药材川贝母的进一步质量控制提供依据.
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| 关 键 词： | 川贝母 质量考察 DNA指纹鉴定技术 |
Quality of Commercially Available Unibract Fritillary Bulb Based on DNA Fingerprint Identification Technology |
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| Zhou Tingting,Li Mingcheng.Quality of Commercially Available Unibract Fritillary Bulb Based on DNA Fingerprint Identification Technology[J].Journal of Beihua University(Natural Science),2017,18(6). |
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| Authors: | Zhou Tingting Li Mingcheng |
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| Abstract: | Objective To investigate the quality of Unibract Fritillary Bulb in different regions. Method The 24 samples of Unibract Fritillary Bulb from 3 cities of China were identified by PCR kit assay and Pharmacopoeia method. Results In the 24 batch of samples of Unibract Fritillary Bulb,there are 18 batch of counterfeit products and 6 batch of genuine,with the pass rate of 25%. In addition,the identification results of the kit assay and the Pharmacopoeia method were the same. Conclusion At present,the quality of commercially available Unibract Fritillary Bulb is not optimistic. The DNA fingerprint identification method is simple and feasible, therefore,the results provide a basis for the further quality control of Chinese herbal medicine. |
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| Keywords: | Unibract Fritillary Bulb quality analysis DNA fingerprint test technology |
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