放线菌酮诱导SV40增强子的启动子活性的研究

在蛋白质合成抑制放线菌酮（CHX）诱导下，pCAT－E质粒中SV40增强子能启动CAT基因的表达，在很短的时间（5min)和很低的CHX质量浓度（30ng/mL）条件下，CHX即能诱导pCAT－E中SV40增强子的启动子活性，为了研究CHX诱导的pCAT-E质粒中SV40增强子的启动子活性是否与位置有关，构建了两个组体：pCAT-Bas-Enh(-)和pCAT-Bas-Enh( ),改变了SV40增强了序列与载体中CAT报告基因的相对位置，用重组质粒转染CHO细胞并用CHX处理，检测不到CAT活性，说明pCAT－E质粒中CHX所诱导的SV40增强子启动子活性受SV40增强子位置的影响。

Cydoheximide Induced Promoter Activity of SV40 Enhancer

The promoter activity of SV40 enhancer in the reporter vector of pCAT-Enhancer was induced by cyclo-heximide (CHX), a common protein synthesis inhibiter. This induced promoter activity can promote the CAT gene expression. The promoter activity of pCAT-Enhancer can be induced after treating 5 mm and 30 ng/mL with CHX. in order to study whether the induced promoter activity of the pCAT-E by CHX is position-dependent. Two recombi-nant DNA has been constructed: pCAT-Bas-Enh (_-) and pCAT-Bas-Enh ( ), in which the position of SV40 enhancer element was changed. _The pCAT-Bas-Enh ( ) / ( - ) plasmids were used to transfect the CHO cell treat-ed with CHX, no CAT activity detected. This result suggests that the CHX induced promoter activity of SV40 en-hancer in pCAT-E is position-dependent.