| 一种从富含次生物质的植物中提取RNA的方法 |
| |
| 引用本文： | 李大力.一种从富含次生物质的植物中提取RNA的方法[J].南京理工大学学报(自然科学版),2001,25(5):547-549. |
| |
| 作者姓名： | 李大力 |
| |
| 作者单位： | 南京理工大学化工学院 |
| |
| 摘 要： | 该实验以草莓为材料，建立了一种适合于提取富含多酚、多糖等次生物质的植物RNA的方法，此方法在酸性条件下用苯酚／氯仿／异戊醇进行抽提去除DNA，并用乙二醇丁醚来除去植物组织提取液中的多糖、多酚等次生物质，得到了质量很好的RNA样品。
|
| 关 键 词： | RNA 提取 植物 次生物质 分子生物学 草莓 |
| 修稿时间： | 2000年11月30 |
The Method of Extracting Total RNA from Plants with Plenty of Secondary Products |
| |
| LiDali.The Method of Extracting Total RNA from Plants with Plenty of Secondary Products[J].Journal of Nanjing University of Science and Technology(Nature Science),2001,25(5):547-549. |
| |
| Authors: | LiDali |
| |
| Abstract: | With strawberry material,one useful method was established for extracting total RNA from the plants with plenty of such secondary products as polyphenols and polymeric carbohydrates.The method uses phenol and chloroform and isopentanol to clear away DNA under the acid prerequisite,and uses ethylenglycol monobutyl ether to clear away polyphenols,polymeric carbohydrates and other secondary products from plant tissues,and then can receive the high quality RNA sample |
| |
| Keywords: | RNA isolation plants secondary product |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
