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中性红染料摄入法检测人肺泡巨噬细胞吞噬活性探讨
引用本文:李铁民,梁再赋,赵慕丽,粟驰.中性红染料摄入法检测人肺泡巨噬细胞吞噬活性探讨[J].辽宁大学学报(自然科学版),1994(1).
作者姓名:李铁民  梁再赋  赵慕丽  粟驰
作者单位:辽宁大学生物系,中国医科大学医学基础部微生物学教研室,锦州铁路中心医院
摘    要:本文作者对中性红染料摄入法的实验条件──肺泡灌洗细胞贴壁时间选择;摄入中性红时间对吞噬活性的影响,及细胞数量时吞噬活性的影响作了探讨.并对肺癌患者肺泡巨噬细胞吞噬活性作了检测.结果表明,此方法用于检测人肺泡巨噬细胞吞噬活性不但指标客观、准确,而且操作简便、省时、微量,适于大量样本检测.

关 键 词:人肺泡巨噬细胞,吞噬活性,中性红染料摄入法

Research About the Neutral Red Dye Uptake Method Used in the Determination of Human Alveolar Macrophages Phagocytosis Activity
Li Tiemin.Research About the Neutral Red Dye Uptake Method Used in the Determination of Human Alveolar Macrophages Phagocytosis Activity[J].Journal of Liaoning University(Natural Sciences Edition),1994(1).
Authors:Li Tiemin
Institution:Li Tiemin Department of Biology,Liaoning University Liang Zaifu Zhao Muli Department of Microbiology,China Medical University Li Chi Jinzhou Center Hospital of Railway Branch
Abstract:In order to investigate human alveolar macrophages phagocytosisactivity from bronchoalveoiar lavage fliud neutral red dye uptake method was used.The various agents affecting this method were determinated and the alveolar macro-phages phagocytosis activity of eight cancer lung patients was detected.The resultsshowed that this is an easy and objective method of examining human alveolarmacrophages phagocytosis activity,and the alveolar macrophages phagocytosis acti-vity of cancer lung patients was significantly lowered than that of the control gro-up(P<0.01).
Keywords:Human alveolar Macrophages  Macrophages phagocytosis activity  Neutral red dye uptake method  
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