铜绿假单胞菌随机扩增DNA多态性基因分型研究

利用微量液体稀释法进行药敏检测和RAPD技术进行基因分型,调查了铜绿假单胞菌在临床各科中的流行情况,以期建立稳定的临床检测系统,在控制院内感染的流行病研究中发挥更大的作用.结果表明耐药谱分6型,Ⅰ型占44.5%,Ⅱ型占18.5%,Ⅲ型占7.4%,Ⅳ型占7.4%,Ⅴ型占11.1%,Ⅵ型(不定型)占11.1%;RAPD分9型,A型占51.9%,B型占7.4%,C型占7.4%,D型占7.4%,E型占7.4%,F型占3.7%,G型占3.7%,H型占7.4%,I型占3.7%.说明基因型A型、耐药谱Ⅰ型的铜绿假单胞菌是此次ICU医院感染暴发流行的菌株,其他型别为非流行相关菌株.比较结果还说明RAPD基因分型的灵敏性、特异性及可靠性优于耐药谱分型,在医院感染流行病学调查中具有较大的应用价值.

Genotyping of Pseudomonas aeruginosa by Using Randomly Amplified Polymorphic DNA

To find out the infection performance of Pseudomonas aeruginosa(PA) in clinical practice of all sorts,and set up a stable clinical monitoring system of cross infection PA strains were grouped by RAPD typing method and microdilution drug-senstive test typing.All the 27 PA specimen collected were divided into 6 types according to different degrees of antibiotics drug-resistance,of which Type Ⅰmaking up 44.5%;TypeⅡ making up 18.5%;Type Ⅲ amounting to 7.4%;Type Ⅳ amounting to 7.4%;Type Ⅴ taking up 11.1%;Type Ⅵ amounting to 11.1%.2 RAPD typing fell into nine different genotypes.The Genotype A amounting to 51.9%;Genotype B,7.4%;Genotype C 7.4%;Genotype D,7.4%;Genotype E,7.4%;Genotype F,(3.7%);Genotype G,3.7%;Genotype H,7.4%;Genotype I,3.7%.PA strains of Genotype A and Drug-Fast Spectrum Ⅰ were the cause of infection in ICU ward,and other strains were not relevant to the infection.The sensitivity of RAPD typing,identification,and reliability obviously prevails over drug-resistance spectrum typing,so it has considerable significance in the stuy of molecular epidemiology.PA strains were highly resistant to Piperacillin,Cefuroxime and Cefuroxime;comparatively sensitive to Carbostyril antibiotics;resistant to amimo sugar antibiotics in some degree and least resistant to Imipenem.