| 巴戟天细胞悬浮培养生产蒽醌类物质的研究 |
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| 引用本文： | 向红,郭勇.巴戟天细胞悬浮培养生产蒽醌类物质的研究[J].华南理工大学学报(自然科学版),1997(2):62-67. |
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| 作者姓名： | 向红 郭勇 |
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| 作者单位： | 华南理工大学生物工程研究所 |
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| 摘 要： | 巴戟天细胞在含NAA0．25mg／L，Kt0．025mg／t,的B5培养基中进行悬浮培养，细胞比生长速率为0．140天^-1，倍增时问为118．8小时，蒽醌含量可达151．27mg／L，为细胞干重的0．81％。巴戟天细胞中蒽醌含量是巴戟天根的2．17倍，蒽醌的比生产速率是巴戟天根的79．3倍。在固体培养愈伤组织中其蒽醌含量和比产率则分别是巴戟天根的6．1倍和209倍。
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| 关 键 词： | 植物细胞培养 蒽醌 巴戟天 |
STUDIES ON THE PRODUCTION OF ANTHRAQUINONE BY PLANT CELL SUSPENSION CULTURE |
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| Xiang Hong,Guo Yong.STUDIES ON THE PRODUCTION OF ANTHRAQUINONE BY PLANT CELL SUSPENSION CULTURE[J].Journal of South China University of Technology(Natural Science Edition),1997(2):62-67. |
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| Authors: | Xiang Hong Guo Yong |
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| Abstract: | Cells from Morinda Officinalis How were suspension cultured on the B 5 medium containing 0.25 mg/L of NAA,0.025 mg/L of kinetin and 30g/L of sucrose.The specific growth rate of the cells was 0.14 day -1 ,the double time for the cell growth was 118 hours.The yield of anthraquinone was 151 mg/L,the content of anthraquinone in the cultured cell was 0.81 g per 100 g of dried cell weight which was 2.17 fold of that in the plant root. The specific production rate of anthraquinone in the cultured cell was 79 fold higher than that in the plant root of Morinda Officinalis How . |
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| Keywords: | plant cell culture anthraquinone Morinda Officinalis How |
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