非水毛细管电泳检测辣椒粉中苏丹红

首次采用非水毛细管电泳法对两种苏丹红染色剂-苏丹红I与苏丹红II进行了分离检测。考察了电泳介质、背景电解质、SDS浓度对分离的影响。当运行缓冲液为100 mmol/L乙酸钠+35 mmol/L SDS的乙腈/甲醇(6:4,v/v)混合溶液时,两种苏丹红化合物能够在15 min内达到基线分离。各组分的峰面积与待测物浓度在0.01~0.5 mg/mL范围内呈良好的线性关系,检测限均为0.005 mg/mL。最终检测到辣椒样品中含有苏丹红II浓度为3.040 mg/g,苏丹红I与苏丹红II在加标辣椒样品中的回收率分别为94.68%和81.00%。

DETECTION OF SUDAN DYES IN CHILI POWDERS BY NONAQUEOUS CAPILLARY ELECTROPHORESIS

Nonaqueous capillary electrophoresis was applied in the separation and determination of Sudan dyes （Sudan Ⅰ and Sudan Ⅱ）. The effects of the electrophoretic medium, background electrolyte and SDS concentration on the separation were investigated. When the running buffer was acetonitrile/methanol （6：4, v/v） containing 100 mmol/L sodium acetate and 35 mmol/L SDS, two Sudan red compounds could be basically separated and detected in 15 min. Excellent linearity was observed in the range from 0.01-0.5 mg/mL for these two analytes, and the detection limits were both 0.005 mg/mL. Finally, 3.040 mg/g of Sudan Ⅱ was found in chili powders, and the recoveries of Sudan Ⅰ and Sudan Ⅱ in spiked chili powders were 94.68%and 81.00%, respectively.