硝基苯高效降解菌群对硝基苯好氧降解

硝基苯为环境有毒有害物质,好氧条件下硝基苯的生物降解主要通过部分还原途径.吡啶甲酸作为副产物出现在硝基苯的部分还原途径中.生物毒性大,阻碍了硝基苯的降解.本实验分离出3株在好氧条件下以硝基苯为惟一碳、氮、能源的菌株,分别鉴定为Streptomyces albidoflavus(微白黄链霉菌)、Rhodotorula mucilaginosa(胶红酵母)和Micrococcus luteus(藤黄微球菌),并借助其完整细胞考查了由上述3株菌混合而成的菌群对硝基苯的好氧降解.结果表明:硝基苯经部分还原生成2-氨基酚,2-氨基酚再进一步开环降解.在2-氨基酚开环过程中,有吡啶甲酸生成,毗啶甲酸被矿化成二氧化碳和水.

Degradation of nitrobenzene by a mixture of nitrobenzene degrading strains under aerobic condition

Nitrobenzene is harmful to the environment. Microorganisms degrade nitrobenzene mainly through the partial reductive pathway under aerobic condition. However, on the partial reductive pathway, picolinic acid emerged as a dead-end product. The formation of picolinic acid impeded the nitrobenzene degradation. The isolation and characterization of three nitrobenzene degrading strains were reported, which were identified as Streptomyces albidoflavus, Rhodotorula mucilaginosa and Micrococcus luteus. The degradation pathway of nitrobenzene by the mixture of three strains was studied. Nitrobenzene was partially reduced to 2-aminophenol and 2-aminophenol was cleaved and degraded. During the degradation of 2-aminophenol, picolinic acid was formed and was mineralized to CO 2 and H 2O to degrade picolinic acid.