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| 基因枪法获得可育抗除草剂转基因小麦 | |
| 引用本文： | 李德森 渠荣达. 基因枪法获得可育抗除草剂转基因小麦[J]. 南开大学学报(自然科学版), 1997, 30(3): 69-75 |
| 作者姓名： | 李德森 渠荣达 |
| 作者单位： | 南开大学生命科学学院!天津，300071(李德森)，NorthCarolinaStateUniversity!Raleigh，NC27695，USA(渠荣达) |
| 摘 要： | 用基因枪法对两个春小麦品种进行了遗传转化，获得１９株独立转化的，抗除草剂Ｂａｓｔａ的小麦植株，其中１５株自交可育，ＰＣＲ和ＤＮＡ印迹检测证实了该基因在转化植株中的表达，转基因及其表达已遗传到子三代，在已检测的７个转基因后代中，有４个植株其抗除草剂性状以单位点，显性性状的孟德尔方式遗传。 |
| 关 键 词： | BAR基因 小麦 基因枪法 抗除草剂 转基因植株 |
FERTILE,HERBICIDE-RESISTANT TRANSGENIC WHEAT PLANTS OBTAINED BY THE BIOLISTIC METHOD |
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| Li Desen. FERTILE,HERBICIDE-RESISTANT TRANSGENIC WHEAT PLANTS OBTAINED BY THE BIOLISTIC METHOD[J]. Acta Scientiarum Naturalium University Nankaiensis, 1997, 30(3): 69-75 | |
| Authors: | Li Desen |
| Abstract: | Nineteen independently transformed, herbicide (Basta )-resistant wheat plant havebeen so far obtained from two spring wheat varieties by the biolistic method, among which15 were self-fertile. PCR assays and Southern blot cionfirmed the presence 0f BAR trans-gene in the transgenic plants. PAT enzyme assays indicated the expression of BAR gene inthose plants. The transgene and its expression has been inherited to the offspring plants.In four of seven transgenic plant lines studied so far, the herbicide-resistance traitinherited as a dominant trait of single locus in a Mendelian mode. To date, the transgeneand its expression has been inherited to the T3 generation. |
| Keywords: | BAR gene Basta wheat |
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