中国西北少数民族东乡族的Y染色体微卫星单倍型分析

运用国际法医学界推荐的"最小单倍型"共7个微卫星位点(DYS19,DYS389Ⅰ,DYS389Ⅱ,DYS390,DYS391,DYS392,DYS393)和1个高多态性微卫星位点DYS385,采用PCR结合银染显色技术,对采自临夏回族自治区东乡县的133个健康男性个体的DNA,进行了Y染色体8个微卫星的等位基因及单倍型分布状况遗传多态性分析.结果显示,东乡族保留着较高的Y-STR遗传多态性,在测出的66个等位基因中共构建了110种单倍型,单倍型多样性为98.9%,表明该系统有较强的个体识别能力.另外,我们的研究可以为东乡族这一穆斯林民族的起源提供有价值的遗传资料.

Human Y-specific STR haplotypes in Dongxiang ethnic group from Northwest China

Eight Y-chromosomal microsatellites have been used to analyze DNA variation in 133 Dongxiang male subjects from the Muslim minority in Northwest Chinese, a set of seven Y-chromosomal STR loci (DYS19, DYS389 Ⅰ, DYS389 Ⅱ, DYS390, DYS391, DYS392, DYS393) with the addition of the biloci marker DYS385 was used to generate male-specific haplotype databases for the Chinese ethnic group samples and PCR products were separated by manual procedures. Allele distribution frequencies and gene diversity value at seven Y-Chromosome STR loci in the Dongxiang population were calculated. The distribution of Y-STR haplotypes is presented. A total of 110 different haplotypes were found, 94 of them being unique. The Y-STR polymorphism in the Northwest Chinese population with an estimated haplotype diversity of 98.9% and a discrimination capacity of 82.7% will be very useful for testing paternal lineages. This work has an important implication on forensic and anthropological studies.