Neurodegeneration changes in primary central nervous system neurons transfected with the Alzheimer-associated neuronal thread protein gene |
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| Authors: | SM de la Monte JR Wands |
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| Institution: | (1) Rhode Island Hospital, Brown University Medical School, 55 Claverick Street, Room 419, Providence (Rhode Island 02903, USA) Fax: +1 401 444 2939, e-mail: delamonte@hotmail.com , US;(2) Department of Medicine, Rhode Island Hospital, Brown Medical School, Providence, (Rhode Island 02903, USA), US |
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| Abstract: | The AD7c-NTP gene is over-expressed in brains with Alzheimer's disease (AD), and increased levels of the corresponding protein
are detectable in cortical neurons, brain tissue extracts, cerebrospinal fluid, and urine beginning early in the course of
AD neurodegeneration. In the present study, we utilized a novel method to transfect post-mitotic primary neuronal cell cultures,
and demonstrated that over-expression of the AD7c-NTP gene causes cell death and neuritic sprouting, two prominent abnormalities
associated with AD. These results provide further evidence that aberrantly increas-ed AD7c-NTP expression may have a role
in AD-type neurodegeneration. In addition, we demonstrate that primary post-mitotic neurons can be efficiently transfected
with conventional recombinant plasmid DNA to evaluate the effects of gene over-expression in relevant in vitro models.
Received 31 January 2001; received after revision 31 March 2001; accepted 4 April 2001 |
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| Keywords: | , Neuronal thread protein, neurodegeneration, primary neuron culture, DNA transfection, |
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